RAIDD/CRADD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00051

Novus Biologicals, part of Bio-Techne
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NBP3-00051-100ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CRADD Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human RAIDD/CRADD (Accession#: P78560; Met1-Glu199)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for RAIDD/CRADD Antibody

Western Blot: RAIDD/CRADD Antibody [NBP3-00051]

Western Blot: RAIDD/CRADD Antibody [NBP3-00051]

Western Blot: RAIDD/CRADD Antibody [NBP3-00051] - Anti-RAIDD/CRADD rabbit polyclonal antibody at 1:500 dilution. Lane A: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 23 kDa (We are unsure as to the identity of these extra bands).
Immunocytochemistry/ Immunofluorescence: RAIDD/CRADD Antibody [NBP3-00051]

Immunocytochemistry/ Immunofluorescence: RAIDD/CRADD Antibody [NBP3-00051]

Immunocytochemistry/Immunofluorescence: RAIDD/CRADD Antibody [NBP3-00051] - Immunofluorescence staining of RAIDD/CRADD in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human RAIDD/CRADD polyclonal antibody (1:5000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
Immunohistochemistry-Paraffin: RAIDD/CRADD Antibody [NBP3-00051]

Immunohistochemistry-Paraffin: RAIDD/CRADD Antibody [NBP3-00051]

Immunohistochemistry-Paraffin: RAIDD/CRADD Antibody [NBP3-00051] - Immunochemical staining of human RAIDD/CRADD in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).
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Applications for RAIDD/CRADD Antibody

Application
Recommended Usage

ELISA

1:25000-1:50000

Immunocytochemistry/ Immunofluorescence

1:1500-1:50000

Immunohistochemistry-Paraffin

1:2500-1:10000

Immunoprecipitation

0.2-1 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRADD

Apoptosis is triggered by activation of initiator caspases upon complex-mediated clustering of the inactive zymogen, as occurs in the caspase-9-activating apoptosome complex. Activation of caspase-2 occurs in a complex that contains the death domain-containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD (1). RAIDD has a dual-domain structure similar to that of FADD. It has an NH2-terminal caspase homology domain that interacts with caspase-2 and a COOH-terminal DD that interacts with RIP. RAIDD is constitutively expressed in many tissues and thus could play a role in regulating apoptosis in mammalian cells (2). In metazoan cells there exists inactive polypeptide precursors (zymogens), each composed of a prodomain, which is cleaved to activate the protease, and a large and small catalytic subunit. The coupling of these proteases to signaling pathways is probably mediated by adaptor molecules that contain protein-protein interaction motifs such as the death domain (3).

Long Name

CASP2 and RIPK1 Domain Containing Adaptor With Death Domain

Alternate Names

RAIDD

Gene Symbol

CRADD

Additional CRADD Products

Product Documents for RAIDD/CRADD Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RAIDD/CRADD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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