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RALBP1 Antibody (OTI11B2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73788

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI11B2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human RALBP1 (NP_006779) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

76 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RALBP1 Antibody (OTI11B2) - Azide and BSA Free

Western Blot: RALBP1 Antibody (OTI11B2)Azide and BSA Free [NBP2-73788]

Western Blot: RALBP1 Antibody (OTI11B2)Azide and BSA Free [NBP2-73788]

Western Blot: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RALBP1.
Immunocytochemistry/ Immunofluorescence: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788]

Immunocytochemistry/ Immunofluorescence: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788]

Immunocytochemistry/Immunofluorescence: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1.
Immunohistochemistry: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788]

Immunohistochemistry: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788]

Immunohistochemistry: RALBP1 Antibody (OTI11B2) - Azide and BSA Free [NBP2-73788] - Staining of paraffin-embedded prostate tissue using anti-RALBP1 mouse monoclonal antibody.

Applications for RALBP1 Antibody (OTI11B2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

4ug/ml

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RALBP1

The receptor interacting protein kinase 1 (RIP1) is essential for the activation of nuclear factor kappaB (NF-kappaB) by tumor necrosis factor alpha (TNFalpha). RIP1 can be polyubiquitinated at Lys 377, which is required for the activation of IkappaB kinase (IKK) and NF-kappaB.

Alternate Names

Dinitrophenyl S-glutathione ATPase, ralA binding protein 1, ralA-binding protein 1,76 kDa Ral-interacting protein, RalBP1, Ral-interacting protein 1, RIP, RIP1, RLIP1, RLIP76DNP-SG ATPase

Gene Symbol

RALBP1

Additional RALBP1 Products

Product Documents for RALBP1 Antibody (OTI11B2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RALBP1 Antibody (OTI11B2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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