RAMP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20966

Novus Biologicals, part of Bio-Techne
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NBP1-20966
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Predicted:

Human (100%), Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all RAMP1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DYQSKRTEGIV corresponding to C-Terminus according to NP_848488.2.

Epitope

C terminus

Specificity

This antibody is expected to recognize isoform 1 (NP_058590.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RAMP1 Antibody

Western Blot: RAMP1 Antibody [NBP1-20966]

Western Blot: RAMP1 Antibody [NBP1-20966]

Western Blot: RAMP1 Antibody [NBP1-20966] - (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for RAMP1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Experiments gave bands at approx. 18 kDa band and 40 kDa band in rat brain lysate after 0.3ug/ml antibody staining. The appearance of a band with much higher molecular weight corresponds to earlier findings in scientific literature with different antibodies (PMID: 11535606). This protein has a calculated MW of 16.9 kDa band according to mouse NP_058590.1 and 16.8 kDa band according to rat NP_113833.1.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAMP1

The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP1) protein, CRLR functions as a CGRP receptor. The RAMP1 protein is involved in the terminal glycosylation, maturation, and presentation of the CGRP receptor to the cell surface.

Long Name

Receptor Activity Modifying Protein 1

Alternate Names

calcitonin receptor-like receptor activity modifying protein 1, Calcitonin-receptor-like receptor activity-modifying protein 1, CRLR activity-modifying protein 1, receptor (calcitonin) activity modifying protein 1, receptor (G protein-coupled) activity modifying protein 1, receptor activity modifying protein 1, receptor activity-modifying protein 1

Entrez Gene IDs

10267 (Human); 51801 (Mouse); 58965 (Rat)

Gene Symbol

RAMP1

UniProt

NP_058590.1 (Mouse)

Additional RAMP1 Products

Product Documents for RAMP1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RAMP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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