Ran Antibody (ARAN1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1082
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Bovine, Xenopus
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # ARAN1
Format
BSA Free
Concentration
2.0 mg/ml
Product Specifications
Immunogen
Denatured recombinant protein (Human). Uniprot accession no. P6826
Epitope
The antibody epitope is located at the C-terminus (amino acids 207-216), which is highly negatively charged.
Reactivity Notes
Cross-reacts with Xenopus laevis. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Nuclear
Specificity
Ran (ARAN1)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Ran Antibody (ARAN1) - BSA Free
![Western Blot: Ran Antibody (ARAN1)BSA Free [NB600-1082]](https://resources.bio-techne.com/images/products/Ran-Antibody-ARAN1-Western-Blot-NB600-1082-img0001.jpg "Western Blot: Ran Antibody (ARAN1)BSA Free [NB600-1082]")
Western Blot: Ran Antibody (ARAN1)BSA Free [NB600-1082]
Western Blot: Ran Antibody (ARAN1) [NB600-1082] - Whole extract of Jurkat cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Ran Clone: ARAN1 and developed using Goat Anti-Mouse IgG-ALP and a NBT/BCIP substrate.Applications for Ran Antibody (ARAN1) - BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:2000
Immunoprecipitation
1:10-1:500
Western Blot
0.5 - 1.0 ug/ml
Application Notes
WB: Predicted molecular weight: 25 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
2.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Ran
Alternate Names
Androgen receptor-associated protein 24, ARA24Gsp1, GTPase Ran, GTP-binding nuclear protein Ran, guanosine triphosphatase Ran, member RAS oncogene family, OK/SW-cl.81, RAN, member RAS oncogene family, RanGTPase, Ras-like protein TC4, Ras-related nuclear protein, TC4
Gene Symbol
RAN
Additional Ran Products
Product Documents for Ran Antibody (ARAN1) - BSA Free
Product Specific Notices for Ran Antibody (ARAN1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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