Ran Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-68870

Novus Biologicals, part of Bio-Techne
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NBP1-68870
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Ran Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-YEHDLEVAQTTALP corresponding to C-Terminus according to NP_006316.1.

Epitope

C-YEHDLEVAQTTALP

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Ran Antibody

Western Blot: Ran Antibody [NBP1-68870]

Western Blot: Ran Antibody [NBP1-68870]

Western Blot: Ran Antibody [NBP1-68870] - Staining of Mouse (A) and Rat (B) Testis lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: Ran Antibody [NBP1-68870]

Immunocytochemistry/ Immunofluorescence: Ran Antibody [NBP1-68870]

Immunocytochemistry/Immunofluorescence: Ran Antibody [NBP1-68870] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Western Blot: Ran Antibody [NBP1-68870]

Western Blot: Ran Antibody [NBP1-68870]

Western Blot: Ran Antibody [NBP1-68870] - Staining of Human Testis lysate with antibody at 1 ug/mL (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Ran Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:8000

Western Blot

0.1 - 1 ug/mL
Application Notes
WB: Approx. 25 kDa band observed in human, mouse and rat testis lysates (calculated MW of 24.4 kDa band according to NP_006316.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ran

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis an

Alternate Names

Androgen receptor-associated protein 24, ARA24Gsp1, GTPase Ran, GTP-binding nuclear protein Ran, guanosine triphosphatase Ran, member RAS oncogene family, OK/SW-cl.81, RAN, member RAS oncogene family, RanGTPase, Ras-like protein TC4, Ras-related nuclear protein, TC4

Entrez Gene IDs

5901 (Human); 19384 (Mouse); 84509 (Rat)

Gene Symbol

RAN

UniProt

NP_006316.1 (Human)

Additional Ran Products

Product Documents for Ran Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Ran Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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