Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RanBP1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human RAN Binding Protein 1 using the numbering given in entry NP_002873.1 (GeneID 5902).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28821644).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RanBP1 Antibody

Western Blot: RanBP1 Antibody [NB100-79814]

Western Blot: RanBP1 Antibody [NB100-79814]

Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP1 antibody NB100-79814 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: RanBP1 Antibody [NB100-79814]

Western Blot: RanBP1 Antibody [NB100-79814]

Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 on HeLa whole cell lysate using NB100-79814. RanBP1 was also immunoprecipitated using rabbit anti-RanBP1 antibody BL1968 NB100-79815 at 5 mcg/0.5 mg lysate.

Applications for RanBP1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:2000

Immunohistochemistry-Paraffin

1:100-1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:5000
Application Notes
IHC-P, IHC application reported in customer review.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using NB100-79814 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RanBP1

Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.

Alternate Names

HTF9A, MGC88701, RAN binding protein 1, Ran-binding protein 1, RanBP1, ran-specific GTPase-activating protein

Entrez Gene IDs

5902 (Human); 19385 (Mouse)

Gene Symbol

RANBP1

UniProt

NP_002873.1 (Human)

Additional RanBP1 Products

Product Documents for RanBP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RanBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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