RanBP3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-74647
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Primate (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 150 and 200 of human RAN binding protein 3 using the numbering given in entry NP_015561.1 (GeneID 8498).
Reactivity Notes
Orangutan (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RanBP3 Antibody
Western Blot: RanBP3 Antibody [NB100-74647]
Western Blot: RanBP3 Antibody [NB100-74647] - Detection of Human and Mouse RanBP3 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RanBP3 antibody NB100-74647 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RanBP3 was also immunoprecipitated by another rabbit anti-RanBP3 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).Immunohistochemistry: RanBP3 Antibody [NB100-74647]
Immunohistochemistry: RanBP3 Antibody [NB100-74647] - Sample: FFPE sections of human ovarian carcinoma (left) and colon carcinoma (right). Antibody: Affinity purified rabbit anti- RanBP3 used at a dilution of 1:200 (1ug/ml) and 1:80 (2.5 ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy aApplications for RanBP3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:250
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RanBP3
Alternate Names
DKFZp586I1520, RAN binding protein 3, ran-binding protein 3, RAN-binding protein-3, RanBP3
Gene Symbol
RANBP3
UniProt
Additional RanBP3 Products
Product Documents for RanBP3 Antibody
Product Specific Notices for RanBP3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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