RanBP3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97450
![Western Blot: RanBP3 Antibody [NBP2-97450]](https://resources.bio-techne.com/images/products/RanBP3-Antibody-Western-Blot-NBP2-97450-img0004.jpg "Western Blot: RanBP3 Antibody [NBP2-97450]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for RanBP3 Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human RanBP3 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for RanBP3 Antibody
Western Blot: RanBP3 Antibody [NBP2-97450]
Western Blot: RanBP3 Antibody [NBP2-97450] - Anti-RanBP3 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 60 kDa![Immunocytochemistry/ Immunofluorescence: RanBP3 Antibody [NBP2-97450]](https://resources.bio-techne.com/images/products/RanBP3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97450-img0001.jpg "Immunocytochemistry/ Immunofluorescence: RanBP3 Antibody [NBP2-97450]")
Immunocytochemistry/ Immunofluorescence: RanBP3 Antibody [NBP2-97450]
Immunocytochemistry/Immunofluorescence: RanBP3 Antibody [NBP2-97450] - Immunofluorescence staining of RanBP3 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RanBP3 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and cytoplasm.![Immunohistochemistry-Paraffin: RanBP3 Antibody [NBP2-97450]](https://resources.bio-techne.com/images/products/RanBP3-Antibody-Immunohistochemistry-Paraffin-NBP2-97450-img0003.jpg "Immunohistochemistry-Paraffin: RanBP3 Antibody [NBP2-97450]")
Immunohistochemistry-Paraffin: RanBP3 Antibody [NBP2-97450]
Immunohistochemistry-Paraffin: RanBP3 Antibody [NBP2-97450] - Immunochemical staining of human RanBP3 in human epididymis with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.Applications for RanBP3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RanBP3
Alternate Names
DKFZp586I1520, RAN binding protein 3, ran-binding protein 3, RAN-binding protein-3, RanBP3
Gene Symbol
RANBP3
Additional RanBP3 Products
Product Specific Notices for RanBP3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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