RanGAP1 Antibody (1E7R7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16697

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16697-100ul
NBP3-16697-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1E7R7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RanGAP1 Antibodies »

Product Summary for RanGAP1 Antibody (1E7R7)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RanGAP1 (P46060). MASEDIAKLAETLAKTQVAGGQLSFKGKSLKLNTAEDAKDVIKEIEDFDSLEALRLEGNTVGVEAARVIAKALEKKSELKRCHWSDMFTGRLRTEIPPAL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RanGAP1 Antibody (1E7R7)

Western Blot: RanGAP1 Antibody (1E7R7) [NBP3-16697]

Western Blot: RanGAP1 Antibody (1E7R7) [NBP3-16697]

Western Blot: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Western blot analysis of extracts of various cell lines, using RanGAP1 Rabbit mAb (NBP3-16697) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: RanGAP1 Antibody (1E7R7) [NBP3-16697]

Immunocytochemistry/ Immunofluorescence: RanGAP1 Antibody (1E7R7) [NBP3-16697]

Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunofluorescence analysis of U-2 OS cells using RanGAP1 Rabbit mAb (NBP3-16697) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RanGAP1 Antibody (1E7R7) [NBP3-16697]

Immunohistochemistry-Paraffin: RanGAP1 Antibody (1E7R7) [NBP3-16697]

Immunohistochemistry-Paraffin: RanGAP1 Antibody (1E7R7) [NBP3-16697] - Immunohistochemistry of paraffin-embedded mouse spinal cord using RanGAP1 Rabbit mAb (NBP3-16697) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Applications for RanGAP1 Antibody (1E7R7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RanGAP1

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.

Long Name

Ran GTPase Activating Protein 1

Alternate Names

Fug1

Gene Symbol

RANGAP1

Additional RanGAP1 Products

Product Documents for RanGAP1 Antibody (1E7R7)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RanGAP1 Antibody (1E7R7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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