RanGAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1384

Novus Biologicals, part of Bio-Techne
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NB100-1384
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all RanGAP1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence ASEDIAKLAETLAK-C corresponding to N-Terminus according to NP_002874.1.

Localization

Cytoplasmic

Specificity

Please note that in mouse, there is a hypothetical protein called "similar to RANGAP1" (XP_139737.2) that is virtually identical.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RanGAP1 Antibody

Western Blot: RanGAP1 Antibody [NB100-1384]

Western Blot: RanGAP1 Antibody [NB100-1384]

Western Blot: RanGAP1 Antibody [NB100-1384] - (0.2ug/ml) of 3T3 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: RanGAP1 Antibody [NB100-1384]

Immunohistochemistry-Paraffin: RanGAP1 Antibody [NB100-1384]

Immunohistochemistry-Paraffin: RanGAP1 Antibody [NB100-1384] - (2.5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for RanGAP1 Antibody

Application
Recommended Usage

Immunohistochemistry

2 - 4 ug/ml

Immunohistochemistry-Paraffin

2 - 4 ug/ml

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.5 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human brain and 3T3 lysates (predicted MW of 68 kDa band according to NP_002874). IHC-P: Human liver shows vesicular staining in the cytoplasm of hepatocytes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RanGAP1

Ran is a small signaling GTPase that is involved in nucleocytoplasmic transport. Two additional functions of animal Ran in the formation of spindle asters and the reassembly of the nuclear envelope in mitotic cells have been recently reported. In contrast to Ras or Rho, Ran is not associated with membranes. Instead, the spatial sequestering of its accessory proteins, the Ran GTPase-activating protein RanGAP 1 and the nucleotide exchange factor RCC1, appears to define the local concentration of RanGTP vs. RanGDP involved in signaling. Mammalian RanGAP 1 is bound to the nuclear pore by a mechanism involving the attachment of small ubiquitin-related modifier protein (SUMO) to its C terminus and the subsequent binding of the SUMOylated domain to the nucleoporin Nup358.

Long Name

Ran GTPase Activating Protein 1

Alternate Names

Fug1

Entrez Gene IDs

5905 (Human); 19387 (Mouse)

Gene Symbol

RANGAP1

UniProt

NP_002874. (Human)

Additional RanGAP1 Products

Product Documents for RanGAP1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RanGAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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