RAP1A Antibody (5F8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37496

Novus Biologicals, part of Bio-Techne
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NBP2-37496
NBP2-37496-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5F8

Format

BSA Free

Concentration

1 mg/ml

View all Rap1A Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human RAP1A expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAP1A Antibody (5F8) - BSA Free

Western Blot: RAP1A Antibody (5F8)BSA Free [NBP2-37496]

Western Blot: RAP1A Antibody (5F8)BSA Free [NBP2-37496]

Western Blot: RAP1A Antibody (5F8) [NBP2-37496] - Western blot analysis using RAP1A mAb against HEK293 (1) and RAP1A (AA: 28-180)-hIgGFc transfected HEK293 (2) cell lysate.
Flow Cytometry: RAP1A Antibody (5F8) - BSA Free [NBP2-37496]

Flow Cytometry: RAP1A Antibody (5F8) - BSA Free [NBP2-37496]

Flow Cytometry: RAP1A Antibody (5F8) [NBP2-37496] - Flow cytometric analysis of A431 cells using RAP1A mouse mAb (green) and negative control (red).
Western Blot: RAP1A Antibody (5F8)BSA Free [NBP2-37496]

Western Blot: RAP1A Antibody (5F8)BSA Free [NBP2-37496]

Western Blot: RAP1A Antibody (5F8) [NBP2-37496] - Western blot analysis using RAP1A mAb against human RAP1A (AA: 28-180) recombinant protein. (Expected MW is 43.2 kDa)
View 1 more images

Applications for RAP1A Antibody (5F8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rap1A

The product of this gene belongs to the family of RAS-related proteins. These proteins share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between RAP proteins and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. The product of this gene counteracts the mitogenic function of RAS because it can interact with RAS GAPs and RAF in a competitive manner. Two transcript variants encoding the same protein have been identified for this gene.

Long Name

Ras-related Protein 1A

Alternate Names

KREV-1, SMGP21

Gene Symbol

RAP1A

Additional Rap1A Products

Product Documents for RAP1A Antibody (5F8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAP1A Antibody (5F8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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