RAP1GAP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-33710

Novus Biologicals, part of Bio-Techne
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NBP1-33710
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RAP1GAP Antibodies »

Product Summary for RAP1GAP Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Rap1GAP. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (83%).

Localization

Golgi apparatus membrane, Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAP1GAP Antibody

Western Blot: RAP1GAP Antibody [NBP1-33710]

Western Blot: RAP1GAP Antibody [NBP1-33710]

Western Blot: RAP1GAP Antibody [NBP1-33710] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rap1GAP antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunohistochemistry-Paraffin: RAP1GAP Antibody [NBP1-33710]

Immunohistochemistry-Paraffin: RAP1GAP Antibody [NBP1-33710]

Immunohistochemistry-Paraffin: RAP1GAP Antibody [NBP1-33710] - Rap1GAP antibody [N2C2], Internal detects Rap1GAP protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain.Rap1GAP antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: RAP1GAP Antibody [NBP1-33710]

Western Blot: RAP1GAP Antibody [NBP1-33710]

Western Blot: RAP1GAP Antibody [NBP1-33710] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE, antibody diluted at 1:500.

Applications for RAP1GAP Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAP1GAP

Rap1GAP, an 85/95kDa protein, is a member of a family of GAP's specific for Rap1 (1). Though to be an important tumor suppressor, Rap1GAP has no sequence homology with other GAPs such as RasGAP, RanGAP and RhoGAP. Purified as a cytosolic and membrane-bound protein, both isoforms consist of two monomers each contain a dimerization and a catalytic domain (2-4). Mutations in the GAP domain of the tumor suppressor tuberin, can cause benign tumor phenotype tuberous sclerosis (5).

Alternate Names

GTPase activating protein 1, RAP1 GTPase activating protein

Gene Symbol

RAP1GAP

Additional RAP1GAP Products

Product Documents for RAP1GAP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAP1GAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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