RAP6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85137

Novus Biologicals, part of Bio-Techne
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NBP1-85137
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RAP6 Antibodies »

Product Summary for RAP6 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNIDEDRLQEIAGAAAENMLGSLLCLPGSGSVLL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAP6 Antibody

Immunocytochemistry/ Immunofluorescence: RAP6 Antibody [NBP1-85137]

Immunocytochemistry/ Immunofluorescence: RAP6 Antibody [NBP1-85137]

Immunocytochemistry/Immunofluorescence: RAP6 Antibody [NBP1-85137] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
RAP6 Antibody Immunohistochemistry-Paraffin: RAP6 Antibody [NBP1-85137] -

Immunohistochemistry-Paraffin: RAP6 Antibody [NBP1-85137] -

Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
RAP6 Antibody Immunohistochemistry-Paraffin: RAP6 Antibody [NBP1-85137] -

Immunohistochemistry-Paraffin: RAP6 Antibody [NBP1-85137] -

Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
View 1 more images

Applications for RAP6 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAP6

GAPex-5 was identified in a two-hybrid screen for interactors of CIP4 (Cdc42 interacting protein 4). GAPex-5 contains a Ras-GAP domain and a VPS9 (vacuolar sorting protein 9 (VPS9) domain. It functions as a GEF for RAB31 to influence GLUT4 trafficking. It has also been found to be involved in the trafficking and ubiquitination of EGFR. Alternate names for GAPex-5 include GTPase-activating protein and VPS9 domain-containing protein 1, Rab5-activating protein 6, GAPVD1, GAPEX5, RAP6, and KIAA1521.

Alternate Names

DKFZP434C212, GAPex-5, GAPEX5RAP6Rab5-activating protein 6, GTPase activating protein and VPS9 domains 1, GTPase-activating protein and VPS9 domain-containing protein 1, KIAA1521MGC138848, MGC138847

Gene Symbol

GAPVD1

Additional RAP6 Products

Product Documents for RAP6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAP6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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