RAP74 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-17974

Novus Biologicals, part of Bio-Techne
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NBP3-17974-0.1ml
NBP3-17974-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RAP74 Antibodies »

Product Summary for RAP74 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LSNKKIYQEEEMPESGAGSEFNRKLREEARRKKYGIVLKEFRPEDQPWLLRVNGKSGRKFKGIKKGGVTENTSYYIFTQCPDGAFEAFPVHNWYNFTPLARHRTLTAEEAEEEWERRNKVLNHFSIMQQRRLKDQDQD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAP74 Antibody

Western Blot: RAP74 Antibody [NBP3-17974]

Western Blot: RAP74 Antibody [NBP3-17974]

Western Blot: RAP74 Antibody [NBP3-17974] - Analysis in control (vector only transfected HEK293T lysate) and RAP74 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: RAP74 Antibody [NBP3-17974]

Immunocytochemistry/ Immunofluorescence: RAP74 Antibody [NBP3-17974]

Immunocytochemistry/Immunofluorescence: RAP74 Antibody [NBP3-17974] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus & cell junctions.
Immunohistochemistry: RAP74 Antibody [NBP3-17974]

Immunohistochemistry: RAP74 Antibody [NBP3-17974]

Immunohistochemistry: RAP74 Antibody [NBP3-17974] - Staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells.

Applications for RAP74 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAP74

Enables several functions, including RNA polymerase II general transcription initiation factor activity; phosphatase activator activity; and promoter-specific chromatin binding activity. Involved in several processes, including positive regulation of transcription by RNA polymerase II; response to virus; and transcription initiation from RNA polymerase II promoter. Located in cell junction and nucleoplasm. Part of transcription factor TFIID complex.

Alternate Names

BTF4, General transcription factor IIF 74 kDa subunit, general transcription factor IIF subunit 1, general transcription factor IIF, polypeptide 1, 74kDa, TF2F1, TFIIF, TFIIF-alpha, Transcription initiation factor IIF subunit alpha, transcription initiation factor RAP74

Gene Symbol

GTF2F1

Additional RAP74 Products

Product Documents for RAP74 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAP74 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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