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RAPGEFL1 Antibody (4G2)

Novus Biologicals, part of Bio-Techne | Catalog # H00051195-M04

Novus Biologicals, part of Bio-Techne
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H00051195-M04

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4G2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

RAPGEFL1 (NP_057423, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEGPEGLGRKQACLAMLLHFLDTYQGLLQEEEGAGHIIKDLYLLIMKDESLYQGLREDTLRLHQLVETVELKIPEENQPPSKQVKPLFRHFRRIDSCLQ

Reactivity Notes

Human. Other species not tested.

Specificity

RAPGEFL1 (4G2)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for RAPGEFL1 Antibody (4G2)

Western Blot: RAPGEFL1 Antibody (4G2) [H00051195-M04]

Western Blot: RAPGEFL1 Antibody (4G2) [H00051195-M04]

Western Blot: RAPGEFL1 Antibody (4G2) [H00051195-M04] - Analysis of RAPGEFL1 expression in IMR-32 (Cat # L008V1).
ELISA: RAPGEFL1 Antibody (4G2) [H00051195-M04]

ELISA: RAPGEFL1 Antibody (4G2) [H00051195-M04]

ELISA: RAPGEFL1 Antibody (4G2) [H00051195-M04] - Detection limit for recombinant GST tagged RAPGEFL1 is approximately 1ng/ml as a capture antibody.

Applications for RAPGEFL1 Antibody (4G2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAPGEFL1

RAPGEFL1, also known as Rap guanine nucleotide exchange factor-like 1, has a 662 amino acid long isoform that is approximately 73kDa, and two shorter isoforms that are 456 amino acids and 511 amino acids, respectively. Research shows that RAPGEFL1 is likely a guanine nucleotide exchange factor, which activates GTPases via the release of GDP and binding of GTP. RAPGEFL1 has also been shown to interact with MLH1.

Alternate Names

Link GEFII, Link guanine nucleotide exchange factor II, Link-GEFII, MGC134798, MGC134799, Rap guanine nucleotide exchange factor (GEF)-like 1, rap guanine nucleotide exchange factor-like 1, RAP guanine-nucleotide-exchange factor (GEF)-like 1

Entrez Gene IDs

51195 (Human)

Gene Symbol

RAPGEFL1

Additional RAPGEFL1 Products

Product Documents for RAPGEFL1 Antibody (4G2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RAPGEFL1 Antibody (4G2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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