Rapsyn Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85537

Novus Biologicals, part of Bio-Techne
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NBP1-85537-25ul
NBP1-85537
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (94%), Rat (95%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rapsyn Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MECCEESMKIALQHGDRPLQALCLLCFADIHRSRGDLETAFPRYDSAMSIMTEIGNRLGQVQALLGVAKCWVARKALDKALDAIE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rapsyn Antibody

Western Blot: Rapsyn Antibody [NBP1-85537]

Western Blot: Rapsyn Antibody [NBP1-85537]

Western Blot: Rapsyn Antibody [NBP1-85537] - (A) HeLa and (B) C2C12 cells were treated with the indicated siRNAs for 2 days and cellular lysates were subjected to Western blot analysis using Rapsyn antibody. GAPDH was used as loading control. Rapsyn protein levels are reduced in NUP88-depleted cells. Image collected and cropped by CiteAb from the following publication (https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1007845) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: Rapsyn Antibody [NBP1-85537]

Immunocytochemistry/ Immunofluorescence: Rapsyn Antibody [NBP1-85537]

Immunocytochemistry/Immunofluorescence: Rapsyn Antibody [NBP1-85537] - Staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: Rapsyn Antibody [NBP1-85537]

Immunohistochemistry-Paraffin: Rapsyn Antibody [NBP1-85537]

Immunohistochemistry-Paraffin: Rapsyn Antibody [NBP1-85537] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
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Applications for Rapsyn Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

Reported in scientific literature (PMID: 30543681)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rapsyn

Rapsyn belongs to a family of proteins that are receptor associated proteins of the synapse. It contains a conserved cAMP-dependent protein kinase phosphorylation site. It is believed to play some role in anchoring or stabilizing the nicotinic acetylcholine receptor at synaptic sites. It may link the receptor to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin. Two splice variants have been identified for this gene.

Alternate Names

43 kDa postsynaptic protein, Acetylcholine receptor-associated 43 kDa protein, CMS1D43 kDa receptor-associated protein of the synapse, CMS1EMGC3597, RAPsyn, receptor-associated protein of the synapse, receptor-associated protein of the synapse, 43kD, RNF205RING finger protein 205

Gene Symbol

RAPSN

Additional Rapsyn Products

Product Documents for Rapsyn Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rapsyn Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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