RAR alpha/NR1B1 Antibody (1C10)

Novus Biologicals, part of Bio-Techne | Catalog # H00005914-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1C10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RAR alpha/NR1B1 Antibodies »

Product Specifications

Immunogen

RARA (NP_000955, 315 a.a. ~ 424 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QLLPLEMDDAETGLLSAICLICGDRQDLEQPDRVDMLQEPLLEALKVYVRKRRPSRPHMFPKMLMKITDLRSISAKGAERVITLKMEIPGSMPPLIQEMLENSEGLDTLS

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear

Specificity

RARA - retinoic acid receptor, alpha

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RAR alpha/NR1B1 Antibody (1C10)

Western Blot: RAR alpha/NR1B1 Antibody (1C10) [H00005914-M02]

Western Blot: RAR alpha/NR1B1 Antibody (1C10) [H00005914-M02]

Western Blot: RAR alpha/NR1B1 Antibody (1C10) [H00005914-M02] - RARA monoclonal antibody (M02), clone 1C10. Analysis of RARA expression in NIH/3T3.
Western Blot: RAR alpha/NR1B1 Antibody (1C10) [H00005914-M02]

Western Blot: RAR alpha/NR1B1 Antibody (1C10) [H00005914-M02]

Western Blot: RAR alpha/NR1B1 Antibody (1C10) [H00005914-M02] - RARA monoclonal antibody (M02), clone 1C10 Analysis of RARA expression in Hela S3 NE.
Proximity Ligation Assay: RAR alpha/NR1B1 Antibody (1C10) [H00005914-M02]

Proximity Ligation Assay: RAR alpha/NR1B1 Antibody (1C10) [H00005914-M02]

Proximity Ligation Assay: RAR alpha/NR1B1 Antibody (1C10) [H00005914-M02] - Analysis of protein-protein interactions between RXRA and RARA. HeLa cells were stained with anti-RXRA rabbit purified polyclonal 1:1200 and anti-RARA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for RAR alpha/NR1B1 Antibody (1C10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAR alpha/NR1B1

Retinoic acid, a metabolite of vitamin A, is necessary for normal organogenesis but acts as a teratogen at high levels during embryonic and fetal development. Retinoic acid functions through its interaction with the nuclear protein, retinoic acid receptor (RAR). RAR belongs to the steroid and thyroid hormone superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. RAR exists as three major subtypes: alpha, beta and gamma.

Long Name

Retinoic Acid Receptor, alpha

Alternate Names

NR1B1, RARA

Entrez Gene IDs

5914 (Human)

Gene Symbol

RARA

OMIM

180240 (Human)

UniProt

NP_000955 (Human)

Additional RAR alpha/NR1B1 Products

Product Documents for RAR alpha/NR1B1 Antibody (1C10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAR alpha/NR1B1 Antibody (1C10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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