RAR alpha/NR1B1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-969
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-SPSLSPSSHRSSPATQSP corresponding to C-Terminus according to NP_000955.1, NP_001138773.1, NP_001019980.1, NP_001138774.1.
Specificity
This antibody is expected to recognize all three reported isoforms (NP_000955.1; NP_001019980.1; NP_001138774.1 ). Please note the epitope is from the mouse/rat sequence. Human sequence is SPSLSPSSNRSSPATHSP and thus highly similar. Reported variants NP_000955.1 and NP_001138773.1 represent identical proteins.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for RAR alpha/NR1B1 Antibody
![Western Blot: RAR alpha/NR1B1 Antibody [NB300-969]](https://resources.bio-techne.com/images/products/RAR-alpha-NR1B1-Antibody-Western-Blot-NB300-969-img0001.jpg "Western Blot: RAR alpha/NR1B1 Antibody [NB300-969]")
Western Blot: RAR alpha/NR1B1 Antibody [NB300-969]
Western Blot: RAR alpha/NR1B1 Antibody [NB300-969] - Staining of Human Breast (A) and Breast cancer (B) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for RAR alpha/NR1B1 Antibody
Application
Recommended Usage
Peptide ELISA
detection limit 1:8000
Western Blot
0.5 - 2 ug/ml
Application Notes
WB: Approx 55kDa band observed in Human Breast and Breast cancer lysates (calculated MW of 50.8kDa according to NP_000955.1and NP_001019980.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RAR alpha/NR1B1
Long Name
Retinoic Acid Receptor, alpha
Alternate Names
NR1B1, RARA
Entrez Gene IDs
5914 (Human)
Gene Symbol
RARA
UniProt
Additional RAR alpha/NR1B1 Products
Product Documents for RAR alpha/NR1B1 Antibody
Product Specific Notices for RAR alpha/NR1B1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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