RASAL1 Antibody

Western Blot: RASAL1 Antibody [NBP1-32776]

Western Blot: RASAL1 Antibody [NBP1-32776] - Protein expression was upregulated in older female crab-eating monkey brain. Image submitted by a verified customer review.

Western Blot: RASAL1 Antibody [NBP1-32776] -

Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 inhibits in vitro anti-CD3 & dendritic cell-peptide induced T-cell activation. a Antigen (OVA323-39)-dependent activation & proliferation of DO11.10 CD4 + cells expressing Flag-tagged Rasal1 compared with control cells at different OVA peptide concentrations (5–10 ng). CTLA-Ig was used as a control. Upper inset shows expression of Flag-tagged Rasal using anti-Flag in blotting (n = 3). b Inhibition of T-cell responses by Rasal1 to increasing anti-CD3 concentrations. Upper inset: effects of Rasal1 at 48 & 72 h post-anti-CD3 activation (n = 3). c Anti-CD3-driven NFATc1/AP1 promoter activity in Jurkat T cells was inhibited by the presence of Flag-tagged Rasal1 (n = 3). d siRNA (esiRNA) knockdown of Rasal1 enhances T-cell proliferation. esiRNA were tested for their knockdown efficiency by western blot (top right panel), esiRNA1 & 2 correspond to 500 nM & 1000 nM concentrations (n = 3). e Contact time between D011.10 CD4+T-cells expressing Flag-Rasal1 (red) or empty vector (blue) & OVA peptide on bone marrow dendritic cells (BMDCs). Dot blot of T cell/DC contact duration time in presence of OVA was unaffected by Flag-tagged Rasal1 or vector transfection (n = 3). Representative plots show mean with ± SD. p-value was calculated by unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: RASAL1 Antibody [NBP1-32776] -

Western Blot: RASAL1 Antibody [NBP1-32776] - TCR complex purification & Rasal1 association with ZAP-70 in the TCR complex. a Analytic gel from tandem affinity purification (TAP) of TCR plus associated proteins captured from TCR-constituted cells (TCR complex) versus TCR-null negative control cells (control) (left panel). Chart showing the list of TCR-associated proteins identified by MS analysis (right upper panel). The structure of Rasal1 (right lower panel). b Co-precipitation of ZAP-70 with TCR from 3A9 T cells. 3A9 cells were stimulated for 10 min with anti-CD3 followed by immunoprecipitation with anti-TCR zeta & blotting with anti-ZAP-70 or anti-zeta. Lane 1: resting T cells; lane 2: anti-CD3-stimulated T cells. c Rasal1 expression is induced with anti-CD3 activation. Relative fold change expression of Rasal1 versus Rasal3 upon anti-CD3 stimulation of CD4+ & CD8 + peripheral T cells. 18 s rRNA served as a control. d Anti-CD3 induces Rasal1 translocation to the membranes of T cells. Stimulation-dependent association of Rasal1 with the membrane (upper panel) versus cytosolic Rasal1 (lower panel). Anti-actin served as a loading control. LAT was used as a marker of membrane enrichment fractionation (n = 3) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: RASAL1 Antibody [NBP1-32776] -

Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 binds to the immune cell tyrosine kinase ZAP-70. a Proximity ligation assay (PLA) of Rasal1 & ZAP-70 in Jurkat T-cells stained with DAPI, anti-Rasal1, & anti-ZAP-70. Positive PLA signal (fluorescent dots) indicates a distance of 30–40 nm between Rasal1 & ZAP-70 in anti-CD3-activated T cells, indicating their close proximity (n = 2). b Anti-Rasal1 co-precipitates ZAP-70 from primary mouse T cells. Lane 1–2 loading controls, lanes 3-4: anti-Rasal1 IP: lanes 2 & 4. Cells were stimulated with anti-CD3 ligation for 5 min vs IgG control ligation in lanes 1 & 3. c Anti-Rasal1 co-precipitates ZAP-70 from transfected Jurkat T cells. Lanes 1: nonligated (i.e., IgG); lane 2: anti-CD3 ligated T cells for 5 min. Lower panel shows similar level of ZAP-70 expression in lysates of (n = 2) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: RASAL1 Antibody [NBP1-32776] -

Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 binds to the immune cell tyrosine kinase ZAP-70. a Proximity ligation assay (PLA) of Rasal1 & ZAP-70 in Jurkat T-cells stained with DAPI, anti-Rasal1, & anti-ZAP-70. Positive PLA signal (fluorescent dots) indicates a distance of 30–40 nm between Rasal1 & ZAP-70 in anti-CD3-activated T cells, indicating their close proximity (n = 2). b Anti-Rasal1 co-precipitates ZAP-70 from primary mouse T cells. Lane 1–2 loading controls, lanes 3-4: anti-Rasal1 IP: lanes 2 & 4. Cells were stimulated with anti-CD3 ligation for 5 min vs IgG control ligation in lanes 1 & 3. c Anti-Rasal1 co-precipitates ZAP-70 from transfected Jurkat T cells. Lanes 1: nonligated (i.e., IgG); lane 2: anti-CD3 ligated T cells for 5 min. Lower panel shows similar level of ZAP-70 expression in lysates of (n = 2) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31641113), licensed under a CC-BY license. Not internally tested by Novus Biologicals.