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RASAL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32776

Novus Biologicals, part of Bio-Techne
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NBP1-32776

Key Product Details

Species Reactivity

Validated:

Human, Monkey

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Proximity Ligation Assay, Western Blot

Cited:

Knockdown Validated, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RASAL1. The exact sequence is proprietary.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31641113). Monkey reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RASAL1 Antibody

Western Blot: RASAL1 Antibody [NBP1-32776]

Western Blot: RASAL1 Antibody [NBP1-32776]

Western Blot: RASAL1 Antibody [NBP1-32776] - Rasal1 inhibits the ERK1/2 pathway, but not p38 pathway. Expression of Rasal1 in T cells inhibits ERK1,2 phosphorylation. Upper and lower panels: two independent experiments where primary mouse T cells were transfected with Flag-tagged Rasal1 and were ligated with anti-CD3 for 5 min followed by blotting with anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204); anti-ERK1/2, anti-ZAP-70 or anti-Flag. Flag-PLK expression served as a negative control (top panel) (n > 2). Upper right panel shows endogenous levels of Rasal1 in activated primary T cells. Lower right panel: histogram showing the inhibition of pERK relative to ERK expression. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-12544-4) licensed under a CC-BY license.
Immunocytochemistry/ Immunofluorescence: RASAL1 Antibody [NBP1-32776]

Immunocytochemistry/ Immunofluorescence: RASAL1 Antibody [NBP1-32776]

Immunocytochemistry/Immunofluorescence: RASAL1 Antibody [NBP1-32776] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Western Blot: RASAL1 Antibody [NBP1-32776]

Western Blot: RASAL1 Antibody [NBP1-32776]

Western Blot: RASAL1 Antibody [NBP1-32776] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE, antibody diluted at 1:1000.

Applications for RASAL1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
RASAL1 antibody validated for WB from a verified customer review. Use in Proximity Ligation Assay reported in scientific literature (PMID:31641113).

Reviewed Applications

Read 2 reviews rated 3.5 using NBP1-32776 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RASAL1

The protein encoded by this gene is member of the GAP1 family of GTPase-activating proteins. These proteins stimulate the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. This particular family member contains domains which are characteristic of the GAP1 subfamily of RasGAP proteins but, in contrast to the other GAP1 family members, this protein is strongly and selectively expressed in endocrine tissues.

Alternate Names

RAS protein activator like 1 (GAP1 like), RASALGAP1 like protein, rasGAP-activating-like protein 1

Entrez Gene IDs

8437 (Human)

Gene Symbol

RASAL1

UniProt

Additional RASAL1 Products

Product Documents for RASAL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RASAL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.