RASEF Antibody (2D5)
Novus Biologicals, part of Bio-Techne | Catalog # H00158158-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 2D5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
RASEF (AAH23566, 631 a.a. ~ 740 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG
Reactivity Notes
Human. Other species not tested.
Specificity
RASEF (2D5)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for RASEF Antibody (2D5)
![Western Blot: RASEF Antibody (2D5) [H00158158-M01]](https://resources.bio-techne.com/images/products/RASEF-Antibody-2D5-Western-Blot-H00158158-M01-img0007.jpg "Western Blot: RASEF Antibody (2D5) [H00158158-M01]")
Western Blot: RASEF Antibody (2D5) [H00158158-M01]
Western Blot: RASEF Antibody (2D5) [H00158158-M01] - RASEF monoclonal antibody (M01), clone 2D5 Analysis of RASEF expression in K-562.![Immunohistochemistry-Paraffin: RASEF Antibody (2D5) [H00158158-M01]](https://resources.bio-techne.com/images/products/RASEF-Antibody-2D5-Immunohistochemistry-Paraffin-H00158158-M01-img0005.jpg "Immunohistochemistry-Paraffin: RASEF Antibody (2D5) [H00158158-M01]")
Immunohistochemistry-Paraffin: RASEF Antibody (2D5) [H00158158-M01]
Immunohistochemistry-Paraffin: RASEF Antibody (2D5) [H00158158-M01] - Analysis of monoclonal antibody to RASEF on formalin-fixed paraffin-embedded human adrenal gland. Antibody concentration 3 ug/ml.
Sandwich ELISA: RASEF Antibody (2D5) [H00158158-M01] - Detection limit for recombinant GST tagged RASEF is approximately 0.3ng/ml as a capture antibody.
Applications for RASEF Antibody (2D5)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: RASEF
Alternate Names
FLJ31614, RAB45, RAB45, member RAS oncogene family, RAS and EF hand domain containing, RAS and EF-hand domain containing, ras and EF-hand domain-containing protein, Ras-related protein Rab-45
Entrez Gene IDs
158158 (Human)
Gene Symbol
RASEF
UniProt
Additional RASEF Products
Product Documents for RASEF Antibody (2D5)
Product Specific Notices for RASEF Antibody (2D5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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