RASGEF1A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85670

Novus Biologicals, part of Bio-Techne
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NBP1-85670
NBP1-85670-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (91%), Rat (93%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RASGEF1A Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HIELDRVSSIYPEDLMQIVSHMDSLDNHRCRGDLTKTYSLEAYDNWFNCLSMLVATEVCRVVKKKHRTRM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RASGEF1A Antibody

Western Blot: RASGEF1A Antibody [NBP1-85670]

Western Blot: RASGEF1A Antibody [NBP1-85670]

Western Blot: RASGEF1A Antibody [NBP1-85670] - Analysis in control (vector only transfected HEK293T lysate) and RASGEF1A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: RASGEF1A Antibody [NBP1-85670]

Immunocytochemistry/ Immunofluorescence: RASGEF1A Antibody [NBP1-85670]

Immunocytochemistry/Immunofluorescence: RASGEF1A Antibody [NBP1-85670] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RASGEF1A Antibody [NBP1-85670]

Immunohistochemistry-Paraffin: RASGEF1A Antibody [NBP1-85670]

Immunohistochemistry-Paraffin: RASGEF1A Antibody [NBP1-85670] - Staining in human cerebral cortex and liver tissues using anti-RASGEF1A antibody. Corresponding RASGEF1A RNA-seq data are presented for the same tissues.
View 2 more images

Applications for RASGEF1A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RASGEF1A

Guanine nucleotide exchange factor (GEF) for KRAS, HRAS, and NRAS (in vitro). Plays a role in cell migration

Alternate Names

CG4853, CG4853 gene product, FLJ37817, RasGEF domain family, member 1A, ras-GEF domain-containing family member 1A

Gene Symbol

RASGEF1A

Additional RASGEF1A Products

Product Documents for RASGEF1A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RASGEF1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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