RASGRP2 Antibody (3D8)

Novus Biologicals, part of Bio-Techne | Catalog # H00010235-M09

Novus Biologicals, part of Bio-Techne
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H00010235-M09
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Key Product Details

Species Reactivity

Human, Canine

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3D8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RASGRP2 Antibodies »

Product Specifications

Immunogen

RASGRP2 (NP_005816, 65 a.a. ~ 164 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GTLDLDKGCTVEELLRGCIEAFDDSGKVRDPQLVRMFLMMHPWYIPSSQLAAKLLHIYQQSRKDNSNSLQVKTCHLVRYWISAFPAEFDLNPELAEQIKE

Specificity

RASGRP2 (3D8)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for RASGRP2 Antibody (3D8)

Western Blot: RASGRP2 Antibody (3D8) [H00010235-M09]

Western Blot: RASGRP2 Antibody (3D8) [H00010235-M09]

Western Blot: RASGRP2 Antibody (3D8) [H00010235-M09] - Analysis of RASGRP2 expression in transfected 293T cell line by RASGRP2 monoclonal antibody (M09), clone 3D8. Lane 1: RASGRP2 transfected lysatE (75.5 KDa). Lane 2: Non-transfected lysate.
ELISA: RASGRP2 Antibody (3D8) [H00010235-M09]

ELISA: RASGRP2 Antibody (3D8) [H00010235-M09]

ELISA: RASGRP2 Antibody (3D8) [H00010235-M09] - Detection limit for recombinant GST tagged RASGRP2 is approximately 0.3ng/ml as a capture antibody.

Applications for RASGRP2 Antibody (3D8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RASGRP2

The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene.

Alternate Names

Calcium and DAG-regulated guanine nucleotide exchange factor I, CalDAG-GEFI, CDC25Lcalcium and diacylglycerol-regulated guanine nucleotide exchange factor I, Cdc25-like protein, F25B3.3 kinase-like protein, guanine exchange factor MCG7, hCDC25L, MCG7, RAS guanyl nucleotide-releasing protein 2, RAS guanyl releasing protein 2 (calcium and DAG-regulated), RAS guanyl-releasing protein 2

Entrez Gene IDs

10235 (Human)

Gene Symbol

RASGRP2

OMIM

605577 (Human)

Additional RASGRP2 Products

Product Documents for RASGRP2 Antibody (3D8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RASGRP2 Antibody (3D8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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