RASSF1 Antibody (OTI2B11) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73786

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B11

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RASSF1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RASSF1 (NP_009113) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RASSF1 Antibody (OTI2B11) - Azide and BSA Free

Western Blot: RASSF1 Antibody (OTI2B11)Azide and BSA Free [NBP2-73786]

Western Blot: RASSF1 Antibody (OTI2B11)Azide and BSA Free [NBP2-73786]

Western Blot: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASSF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASSF1.
Immunocytochemistry/ Immunofluorescence: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786]

Immunocytochemistry/ Immunofluorescence: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786]

Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RASSF1.
Immunohistochemistry: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786]

Immunohistochemistry: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786]

Immunohistochemistry: RASSF1 Antibody (OTI2B11) - Azide and BSA Free [NBP2-73786] - Staining of paraffin-embedded Human lymphoma tissue using anti-RASSF1 mouse monoclonal antibody.
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Applications for RASSF1 Antibody (OTI2B11) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RASSF1

The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability. Agathanggelou A. et al

Alternate Names

123F2, NORE2A, pancreas-specific ras association domain family 1 protein, Ras association (RalGDS/AF-6) domain family member 1, ras association domain-containing protein 1, RASSF1A, RDA32cardiac-specific ras association domain family 1 protein, REH3P21, tumor suppressor protein RDA32, WUGSC:H_LUCA12.5

Gene Symbol

RASSF1

Additional RASSF1 Products

Product Documents for RASSF1 Antibody (OTI2B11) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RASSF1 Antibody (OTI2B11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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