RASSF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97636

Novus Biologicals, part of Bio-Techne
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NBP2-97636-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RASSF1 Antibodies »

Product Summary for RASSF1 Antibody

Immunogen

Produced in rabbits immunized with E. coli-derived Human RASSF1 fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for RASSF1 Antibody

Immunocytochemistry/ Immunofluorescence: RASSF1 Antibody [NBP2-97636]

Immunocytochemistry/ Immunofluorescence: RASSF1 Antibody [NBP2-97636]

Immunocytochemistry/Immunofluorescence: RASSF1 Antibody [NBP2-97636] - Immunofluorescence staining of RASSF1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RASSF1 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.
Immunohistochemistry-Paraffin: RASSF1 Antibody [NBP2-97636]

Immunohistochemistry-Paraffin: RASSF1 Antibody [NBP2-97636]

Immunohistochemistry-Paraffin: RASSF1 Antibody [NBP2-97636] - Immunochemical staining of human RASSF1 in human adrenal gland with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Applications for RASSF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RASSF1

The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability. Agathanggelou A. et al

Alternate Names

123F2, NORE2A, pancreas-specific ras association domain family 1 protein, Ras association (RalGDS/AF-6) domain family member 1, ras association domain-containing protein 1, RASSF1A, RDA32cardiac-specific ras association domain family 1 protein, REH3P21, tumor suppressor protein RDA32, WUGSC:H_LUCA12.5

Gene Symbol

RASSF1

Additional RASSF1 Products

Product Documents for RASSF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RASSF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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