RASSF8 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88388

Novus Biologicals, part of Bio-Techne
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NBP1-88388
NBP1-88388-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RASSF8 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KQLKDQLQEIRQKITECENKLKDYLAQIRTMESGLEAEKLQREVQEAQVNEEEVKGKIGKVKGEIDIQGQQSLRLENGIKAVERSLGQATKRLQDKEQE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (84%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RASSF8 Antibody

Western Blot: RASSF8 Antibody [NBP1-88388]

Western Blot: RASSF8 Antibody [NBP1-88388]

Western Blot: RASSF8 Antibody [NBP1-88388] - Analysis in human cell lines PC-3 and A-549 using anti-RASSF8 antibody. Corresponding RASSF8 RNA-seq data are presented for the same cell lines. Loading control: anti-COX4I1.
Immunocytochemistry/ Immunofluorescence: RASSF8 Antibody [NBP1-88388]

Immunocytochemistry/ Immunofluorescence: RASSF8 Antibody [NBP1-88388]

Immunocytochemistry/Immunofluorescence: RASSF8 Antibody [NBP1-88388] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & mitochondria.
Immunohistochemistry-Paraffin: RASSF8 Antibody [NBP1-88388]

Immunohistochemistry-Paraffin: RASSF8 Antibody [NBP1-88388]

Immunohistochemistry-Paraffin: RASSF8 Antibody [NBP1-88388] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
View 1 more images

Applications for RASSF8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RASSF8

Alternate Names

C12orf2, carcinoma associated HOJ-1, Carcinoma-associated protein HOJ-1, chromosome 12 open reading frame 2, DKFZp434O0227, FLJ11542, HOJ1, HoJ-1, Ras association (RalGDS/AF-6) domain family (N-terminal) member 8, ras association domain-containing protein 8

Gene Symbol

RASSF8

Additional RASSF8 Products

Product Documents for RASSF8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RASSF8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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