Rat GABAB R2 N-Terminus Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1188
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat GABAB R2
Trp41-Ser482 (Phe337Tyr)
Accession # O88871
Trp41-Ser482 (Phe337Tyr)
Accession # O88871
Specificity
Detects rat GABAB R2 N-Terminus in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rat GABAB R2 N-Terminus Antibody
GABABR2 in Rat Spinal Cord.
GABABR2 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GABABR2 N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1188) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal horn. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Rat GABAB R2 by Simple WesternTM.
Simple Western lane view shows lysates of rat brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GABAB R2 at approximately 148 kDa (as indicated) using 20 µg/mL of Goat Anti-Rat GABAB R2 N-Terminus Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1188) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Applications for Rat GABAB R2 N-Terminus Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (cortex) and spinal cord
Sample: Perfusion fixed frozen sections of rat brain (cortex) and spinal cord
Simple Western
20 µg/mL
Sample: Rat brain tissue
Sample: Rat brain tissue
Western Blot
0.1 µg/mL
Sample: Recombinant Rat GABAB R2 N-Terminus
Sample: Recombinant Rat GABAB R2 N-Terminus
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GABA-B R2
Long Name
gamma-Aminobutyric Acid Type B Receptor Subunit 2
Alternate Names
GABABR2, GABBR2, GPR51, GPRC3B
Gene Symbol
GABBR2
UniProt
Additional GABA-B R2 Products
Product Documents for Rat GABAB R2 N-Terminus Antibody
Product Specific Notices for Rat GABAB R2 N-Terminus Antibody
For research use only
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