Rat GFR alpha-1/GDNF R alpha-1 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF560

R&D Systems, part of Bio-Techne
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BAF560
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AF560
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Mouse, Avian - Chicken

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all GFR alpha-1/GDNF R alpha-1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat GFR alpha‑1/GDNF R alpha‑1
Asp25-Leu445
Accession # Q62997

Specificity

Detects rat GFR alpha‑1/GDNF R alpha‑1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat GFR alpha-1/GDNF R alpha-1 Biotinylated Antibody

GDNF and GFRa-1 antibody in Rat Dorsal Root Ganglia by Immunohistochemistry (IHC-Fr).

GDNF and GFR alpha-1 in Rat Dorsal Root Ganglia.

GDNF and GFRa-1 were detected in perfusion fixed frozen sections of rat dorsal root ganglia using 10 µg/mL Human/Rat GDNF Antigen Affinity-purified Polyclonal Antibody (green; Catalog # AF-212-NA) and 15 µg/mL Goat AntiRat Anti-GFRa-1 Biotinylated Antigen Affinity-purified Poly-clonal Antibody (red; Catalog # BAF560). Tissue was incubated with primary antibodies overnight at 4 °C. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Rat GFR alpha-1/GDNF R alpha-1 Biotinylated Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (cortex) and rat dorsal root ganglia

Western Blot

0.1 µg/mL
Sample: Recombinant Rat GFR alpha-1/GDNF R alpha-1 Fc Chimera (Catalog # 560-GR)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-1/GDNF R alpha-1

Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha- (GFR alpha-1) or GFR alpha-2). GFR alpha-1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha-1 or GFR alpha-2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alphas released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha-1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha-1 cDNA encodes a 468 amino acid (aa) residue protein with an N‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha-1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha-1 shares 93% amino acid identity with rat GFR alpha-1. The expression of the various GFR alphas are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alphas in mediating the activities of the GDNF family of neurotrophic factors.

References

  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha 1

Alternate Names

GDNF R alpha-1, GFR alpha1, GFRa-1, GFRA1

Entrez Gene IDs

2674 (Human); 14585 (Mouse); 25454 (Rat)

Gene Symbol

GFRA1

UniProt

Q62997

Additional GFR alpha-1/GDNF R alpha-1 Products

Product Documents for Rat GFR alpha-1/GDNF R alpha-1 Biotinylated Antibody

Product Datasheets

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Product Specific Notices for Rat GFR alpha-1/GDNF R alpha-1 Biotinylated Antibody

For research use only

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