Rat mGluR1 C-Terminus Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS077

R&D Systems, part of Bio-Techne
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PPS077
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Key Product Details

Species Reactivity

Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all mGluR1 Antibodies »

Product Specifications

Immunogen

Peptide from the C-terminus region of rat mGluR1a

Specificity

Rat ~125 kDa and ~250 kDa dimer mGluR1a

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rat mGluR1 C-Terminus Antibody

Detection of mGluR1 C-Terminus antibody by Western Blot

Detection of mGluR1 C-Terminus by Western Blot

Western blot of HEK 293 cells expressing mGluR1a and mGluR5 showing the specific immunolabeling of the ~125 kDa monomer and the ~250 kDa dimer of mGluR1a.

Applications for Rat mGluR1 C-Terminus Antibody

Application
Recommended Usage

Immunohistochemistry

(fozen sections; unpublished observations)

Western Blot

1:1000 dilution
Sample: HEK293 human embryonic kidney cell line expressing mGluR1a

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: mGluR1

Rat metabotropic glutamate receptor 1 (mGluR1a; also mGlu1a) is a 140 kDa, 1181 amino acid (aa), 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. Group I receptors include mGluR1 and mGluR5. Both are postsynaptic, associated with Gq-like proteins, mobilize Ca++ from intracellular stores, and regulate neuronal excitability by influencing ion channel activity. Their activities are not necessarily redundant. When co-expressed on select neurons, mGluR5 tends to dampen mGluR1 activity. On T cells, mGluR1 is inducible and promotes cell proliferation, while mGluR5 is constitutive and blocks proliferation. mGluR1 has a large glycosylated extracellular domain (ECD) of 574 aa. The ECD either covalently homodimerizes or heterodimerizes with the Ca++-sensor receptor (CaSR). Dimerization creates two subunit-linked “open clamshells” which first bind glutamate, then close, and subsequently undergo rearrangement for signal transduction. There are two alternate splice forms of rat mGluR1. Each shows a short aa substitution and premature truncation after N887 in the cytoplasmic region. The cytoplasmic, C-terminal 310 amino acids of rat mGluR1a are 90% and 97% aa identical to the analogous amino acids in human and mouse mGluR1a, respectively.

References

  1. Pin, J-P. and F. Acher (2002) Curr. Drug Targets CNS Neurol. Disord. 1:297.
  2. Boldyrev, A.A. et al. (2005) J. Neurochem. 95:913.
  3. Poisik, O.V. et al. (2003) J. Neurosci. 23:122.
  4. Ferraguti, F. and R. Shigemoto (2006) Cell Tissue Res. 326:483.
  5. Gama, L. et al. (2001) J. Biol. Chem. 276:39053.

Long Name

Metabotropic Glutamate Receptor 1

Alternate Names

GPRC1A, GRM1

Entrez Gene IDs

2911 (Human); 14816 (Mouse); 24414 (Rat)

Gene Symbol

GRM1

Additional mGluR1 Products

Product Documents for Rat mGluR1 C-Terminus Antibody

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Product Specific Notices for Rat mGluR1 C-Terminus Antibody

For research use only

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