Rat mGluR2/3 Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS078

R&D Systems, part of Bio-Techne
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PPS078
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all mGluR2/3 Antibodies »

Product Specifications

Immunogen

Peptide from the C-terminus region of rat mGluR2 and mGluR3

Specificity

Rat ~110 kDa mGluR2 and mGluR3 monomers and ~220 kDa homodimers

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rat mGluR2/3 Antibody

Detection of mGluR2/3 antibody by Western Blot

Detection of mGluR2/3 by Western Blot

Western blot of rat hippocampal lysate showing the specific immunolabeling of the ~110 kDa monomer and the ~220 kDa dimer of mGluR2 and mGluR3.

Applications for Rat mGluR2/3 Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat brain (hippocampus) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA, and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: mGluR2/3

Rat metabotropic glutamate receptor 2 (mGluR2; also mGlu2) is a 95 kDa, 854 amino acid (aa), 7-transmembrane glycoprotein that belongs to group II of the C-family of G-protein coupled receptors. Group II receptors include mGluR2 and mGluR3. Both are presynaptic, considered to negatively regulate the release of glutamate, associate with Gi/Go proteins, and inhibit adenylate cyclase, thus decreasing cAMP concentration. The effects of mGluR2 and 3 are not always identical. On microglia, mGluR2 but not mGluR3 stimulation induces TNF-alpha release. mGluR2 is believed to exist as a dimer. Dimerization presumably creates two subunit-linked “open clamshells” which first bind glutamate, then close, and subsequently undergo rearrangement for signal transduction. The cytoplasmic tails of rat mGluR2 and mGluR3 are 64% aa identical; over the last 18 aa, they are 95% aa identical.

References

  1. Rudd, M.T. and J.A. Mccauley (2005) Curr. Top. Med. Chem. 5:869.
  2. Gasparini, F. et al. (2002) Curr. Opin. Pharmacol. 2:43.
  3. Ferraguti, F. and R. Shigemoto (2006) Cell Tissue Res. 326:483.
  4. Shen, K-Z. and S.W. Johnson (2003) J. Physiol. 533:489.
  5. Taylor, D.L. et al. (2005) J. Neurosci. 25:2952.

Long Name

Metabotropic Glutamate Receptor 2 and 3

Alternate Names

GLUR2, glutamate metabotropic receptor 2, glutamate receptor, metabotropic 2, GPRC1Bmetabotropic glutamate receptor 2, mGlu2, mGluR2, MGLUR2glutamate receptor homolog

Additional mGluR2/3 Products

Product Documents for Rat mGluR2/3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Rat mGluR2/3 Antibody

For research use only

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