RB1 Antibody (S09-4H8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19971

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S09-4H8

Concentration

0.3 mg/ml

View all RB1 Antibodies »

Product Summary for RB1 Antibody (S09-4H8)

Immunogen

A synthetic peptide of human RB1 (Uniprot # P06400)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RB1 Antibody (S09-4H8)

Western Blot: RB1 Antibody (S09-4H8) [NBP3-19971]

Western Blot: RB1 Antibody (S09-4H8) [NBP3-19971]

Western Blot: RB1 Antibody (S09-4H8) [NBP3-19971] - Western blot detection of RB1 in 3T3 cell lysates using NBP3-19971 (1:1000 diluted). Predicted band size: 106kDa. Observed band size: 110kDa.
Immunocytochemistry/ Immunofluorescence: RB1 Antibody (S09-4H8) [NBP3-19971]

Immunocytochemistry/ Immunofluorescence: RB1 Antibody (S09-4H8) [NBP3-19971]

Immunocytochemistry/Immunofluorescence: RB1 Antibody (S09-4H8) [NBP3-19971] - Immunocytochemistry of RB1 (green) in Hela cells using NBP3-19971 at dilution 1/200, and DAPI (blue)

Applications for RB1 Antibody (S09-4H8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50-1/200

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RB1

Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of ts function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia.

Long Name

Retinoblastoma-associated protein

Alternate Names

OSRC, p105-Rb, pp110

Gene Symbol

RB1

Additional RB1 Products

Product Documents for RB1 Antibody (S09-4H8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RB1 Antibody (S09-4H8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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