RB1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20127

Novus Biologicals, part of Bio-Techne
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NBP2-20127
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (97%), Porcine (96%), Rat (96%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RB1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RB1. The exact sequence is proprietary.

Reactivity Notes

Chicken (82%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

106 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RB1 Antibody

Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127]

Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127]

Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127] - Mouse eye. Green: Rb stained by Rb antibody diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunocytochemistry/ Immunofluorescence: RB1 Antibody [NBP2-20127]

Immunocytochemistry/ Immunofluorescence: RB1 Antibody [NBP2-20127]

Immunocytochemistry/Immunofluorescence: RB1 Antibody [NBP2-20127] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rb stained by Rb antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 um.
Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127]

Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127]

Immunohistochemistry-Paraffin: RB1 Antibody [NBP2-20127] - Human colon carcinoma. Rb antibody dilution: 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
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Applications for RB1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RB1

The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq]

Long Name

Retinoblastoma-associated protein

Alternate Names

OSRC, p105-Rb, pp110

Gene Symbol

RB1

Additional RB1 Products

Product Documents for RB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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