RBBP4/RbAp48 Antibody (13D10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41201

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-41201
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

Block/Neutralize, Chemotaxis, Chromatin Immunoprecipitation (ChIP), IF/IHC, In vivo assay, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 13D10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RBBP4 Antibodies »

Product Specifications

Immunogen

The complete coding region (amino acids 1-425) of RBBP4/RbAp48 expressed in E. coli.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34205080).

Localization

Nuclear

Specificity

This antibody will recognize human RbAp48.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RBBP4/RbAp48 Antibody (13D10)

Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201]

Immunocytochemistry/ Immunofluorescence: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201]

Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RbAp48 protein stained by RbAp48 antibody [13D10] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201]

Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201]

Western Blot: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Detection of RbAp48 in HeLa nuclear extract
Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201]

Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201]

Immunoprecipitation: RBBP4/RbAp48 Antibody (13D10) [NBP1-41201] - Immunoprecipitation of RbAp48 protein from HeLa whole cell extract using 5 ug of RbAp48 antibody [13D10]. Western blot analysis was performed using RbAp48 antibody [13D10]. EasyBlot HRP-conjugated anti mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
View 1 more images

Applications for RBBP4/RbAp48 Antibody (13D10)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Reactivity reported in (PMID: 30551478)

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

Assay dependent

Immunoprecipitation

1:500

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP1-41201 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBBP4

RbAp48-13D10 recognizes full-length RbAp48, a 48a nuclear protein first isolated by its ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). It is a member of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1.

Long Name

Retinoblastoma Binding Protein 4

Alternate Names

CAF-1 p48, CAF-1C, NURF55, RBAP48

Entrez Gene IDs

5928 (Human)

Gene Symbol

RBBP4

Additional RBBP4 Products

Product Documents for RBBP4/RbAp48 Antibody (13D10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RBBP4/RbAp48 Antibody (13D10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.