RbBP5 Antibody (PSH0-15)

Western Blot: RbBP5 Antibody (PSH0-15) [NBP3-32895]

Western Blot: RbBP5 Antibody (PSH0-15) [NBP3-32895] - Western blot analysis of RbBP5 on different lysates with Rabbit anti-RbBP5 antibody (NBP3-32895) at 1/1,000 dilution.

Lane 1: SW480 cell lysate
Lane 2: A431 cell lysate
Lane 3: C2C12 cell lysate
Lane 4: 293 cell lysate
Lane 5: MCF-7 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: K562 cell lysate
Lane 8: N2A cell lysate
Lane 9: PC-12 cell lysate
Lane 10: RAW264.7 cell lysate

Lysates/proteins at 25 ug/Lane.

Predicted band size: 59 kDa
Observed band size: 59 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32895) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.