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RbBP5 Antibody (PSH0-16)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32896

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-32896

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # PSH0-16

Concentration

1 mg/ml

Product Specifications

Immunogen

Synthetic peptide within human RbBP5 aa 431-480 / 538. (Uniprot: Q15291)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RbBP5 Antibody (PSH0-16)

RbBP5 Antibody (PSH0-16)

Immunohistochemistry: RbBP5 Antibody (PSH0-16) [NBP3-32896]

Immunohistochemistry: RbBP5 Antibody (PSH0-16) [NBP3-32896] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-RbBP5 antibody (NBP3-32896) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32896) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for RbBP5 Antibody (PSH0-16)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH7.4), 0.1% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RbBP5

RbBP5 is encoded by this gene is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B.

Alternate Names

RBQ3RBBP-5, retinoblastoma binding protein 5, Retinoblastoma-binding protein RBQ-3, Set1c WD40 repeat protein, homolog, SWD1

Gene Symbol

RBBP5

Additional RbBP5 Products

Product Documents for RbBP5 Antibody (PSH0-16)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RbBP5 Antibody (PSH0-16)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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