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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 500 and the C-terminus (residue 538) of human retinoblastoma binding protein 5 using the numbering given in entry NP_005048.2 (GeneID 5929).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RbBP5 Antibody

Western Blot: RbBP5 Antibody [NB600-252]

Western Blot: RbBP5 Antibody [NB600-252]

Western Blot: RbBP5 Antibody [NB600-252] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RbBP5 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: RbBP5 Antibody [NB600-252]

Immunohistochemistry-Paraffin: RbBP5 Antibody [NB600-252]

Immunohistochemistry-Paraffin: RbBP5 Antibody [NB600-252] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- RbBP5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Chromatin Immunoprecipitation Sequencing: RbBP5 Antibody [NB600-252] - Localization of RbBP5 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-RbBP5 antibody NB600-252 and analyzed by DNA sequencing. The figure illustrates the peak distribution of RbBP5 binding within a 500 Kb region of chromosome 1 as detected using anti-RbBP5 antibody NB600-252. ChIP-seq validation performed by Diogenode, Denville, NJ.

Applications for RbBP5 Antibody

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

10 ug

Chromatin Immunoprecipitation Sequencing

2 ug

Immunocytochemistry/ Immunofluorescence

1-4 ug/ml

Immunohistochemistry

1:500- 1:2000

Immunohistochemistry-Paraffin

1:500- 1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:10000 - 1:25000
Application Notes
Reported use in ChIP assays (see Dou et al). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 5 using NB600-252 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RbBP5

The protein encoded by this gene is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. It is found among several proteins that bind directly to retinoblastoma protein, which regulates cell proliferation. The encoded protein interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B.

Alternate Names

RBQ3RBBP-5, retinoblastoma binding protein 5, Retinoblastoma-binding protein RBQ-3, Set1c WD40 repeat protein, homolog, SWD1

Entrez Gene IDs

5929 (Human); 213464 (Mouse)

Gene Symbol

RBBP5

UniProt

Additional RbBP5 Products

Product Documents for RbBP5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RbBP5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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