RBBP6 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49535

Novus Biologicals, part of Bio-Techne
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NBP1-49535
NBP1-49535SS
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all RBBP6 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human RBBP6 protein (between residues 1600-1650) [UniProt Q7Z6E9]

Localization

Nucleus nucleolus. Chromosome. Note: Colocalizes with mitotic chromosomes.

Specificity

This antibody may detect isoform 1 (201.6 kDa), isoform 2 (197.3 kDa) and isoform 4 (106.0 kDa) of human RBBP6.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RBBP6 Antibody - BSA Free

Western Blot: RBBP6 AntibodyBSA Free [NBP1-49535]

Western Blot: RBBP6 AntibodyBSA Free [NBP1-49535]

Western Blot: RBBP6 Antibody [NBP1-49535] - Analysis of RbBP6 in HeLa nuclear extracts.
Immunohistochemistry-Paraffin: RBBP6 Antibody - BSA Free [NBP1-49535]

Immunohistochemistry-Paraffin: RBBP6 Antibody - BSA Free [NBP1-49535]

Immunohistochemistry-Paraffin: RBBP6 Antibody [NBP1-49535] - IHC analysis of formalin-fixed paraffin-embedded tissue section from intestine of normal mouse with 1:400 dilution of RBBP6 antibody. The intestinal epithelial cells depicted a nuclear and cytoplasmic immunostaining pattern with this antibody.
Immunohistochemistry-Paraffin: RBBP6 Antibody - BSA Free [NBP1-49535]

Immunohistochemistry-Paraffin: RBBP6 Antibody - BSA Free [NBP1-49535]

Immunohistochemistry-Paraffin: RBBP6 Antibody [NBP1-49535] - IHC analysis of formalin-fixed paraffin-embedded tissue section from skin of normal mouse with 1:400 dilution of RBBP6 antibody. The keratinocytes and other epidermal cells developed nuclear and cytoplasmic immunostaining with this antibody.
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Applications for RBBP6 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 27667193)

Immunohistochemistry-Paraffin

1:400

Immunoprecipitation

1:10-1:500

Knockdown Validated

reported in scientific literature (PMID 34348893)

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBBP6

RBBP6 (retinoblastoma binding protein 6; P2PR, PACT, RBQ1) is a multidomain protein with an expected molecular size of about 250kD and it colocalizes with mitotic chromosomes as well as with NEK6 in centrosomes. RBBP6 is highly expressed in normal placenta and testis, and is overexpressed in certain cancers (esophageal and lung cancer). RBBP6 is a multifunctional protein which is found presumably in all eukaryotes and is implicated in versatile cellular activities such as mRNA metabolism, cell cycle regulation, carcinogenesis, and development. It forms part of the pre-mRNA 3-end processing complex in humans and the cleavage/polyadenylation factor complex in yeast. RBBP6 interacts with p53/TP53, RB1, NEK6, MDM2, YBX1 etc., and gets phosphorylated upon DNA damage via ATM/ATR or through NEK6. RBBP6 is an E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, thereby facilitating its proteasomal degradation. RBBP6 also acts as a scaffold protein to enhance the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53, thereby acting as a negative regulator of p53/TP53.

Alternate Names

E3 ubiquitin-protein ligase RBBP6, EC 6.3.2.-, MY038, P2PR, P2P-R, p53-associated cellular protein of testis, PACTDKFZp761B2423, Proliferation potential-related protein, Protein P2P-R, RB-binding Q-protein 1, RBQ1, RBQ-1, retinoblastoma binding protein 6, Retinoblastoma-binding protein 6DKFZp686P0638, Retinoblastoma-binding Q protein 1, SNAMA

Entrez Gene IDs

5930 (Human)

Gene Symbol

RBBP6

UniProt

Q7Z6E9 (Human)

Additional RBBP6 Products

Product Documents for RBBP6 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RBBP6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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