RBJ Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80857
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Mouse (93%), Rat (91%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GGKRPTTNSSASFTKEQADAIRRIRNSKDSWDMLGVKPGASRDEVNKAYRKLAVLLHPDKCVAPGSEDAFKAVVNARTALLK
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RBJ Antibody
Western Blot: RBJ Antibody [NBP1-80857]
Western Blot: RBJ Antibody [NBP1-80857] - Analysis in control (vector only transfected HEK293T lysate) and DNAJC27 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: RBJ Antibody [NBP1-80857]
Immunohistochemistry-Paraffin: RBJ Antibody [NBP1-80857] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.Applications for RBJ Antibody
Application
Recommended Usage
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RBJ
Alternate Names
DKFZp434N211, DnaJ (Hsp40) homolog, subfamily C, member 27, rab and DnaJ domain containing, Rab and DnaJ domain-containing protein, RABJS, Ras-associated protein Rap1, RBJdnaJ homolog subfamily C member 27
Entrez Gene IDs
51277 (Human)
Gene Symbol
DNAJC27
UniProt
Additional RBJ Products
Product Documents for RBJ Antibody
Product Specific Notices for RBJ Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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