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RBJ Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80857

Novus Biologicals, part of Bio-Techne
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NBP1-80857
NBP1-80857-25ul

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (93%), Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GGKRPTTNSSASFTKEQADAIRRIRNSKDSWDMLGVKPGASRDEVNKAYRKLAVLLHPDKCVAPGSEDAFKAVVNARTALLK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RBJ Antibody

Western Blot: RBJ Antibody [NBP1-80857]

Western Blot: RBJ Antibody [NBP1-80857]

Western Blot: RBJ Antibody [NBP1-80857] - Analysis in control (vector only transfected HEK293T lysate) and DNAJC27 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: RBJ Antibody [NBP1-80857]

Immunohistochemistry-Paraffin: RBJ Antibody [NBP1-80857]

Immunohistochemistry-Paraffin: RBJ Antibody [NBP1-80857] - Staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Applications for RBJ Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBJ

RBJ (DnaJ homolog subfamily C member 27, DNAJC27) is a 31 kDa member of the small GTPase superfamily. It may lack GTPase activity. It may play a role in ovarian hyperstimulation syndrome.

Alternate Names

DKFZp434N211, DnaJ (Hsp40) homolog, subfamily C, member 27, rab and DnaJ domain containing, Rab and DnaJ domain-containing protein, RABJS, Ras-associated protein Rap1, RBJdnaJ homolog subfamily C member 27

Entrez Gene IDs

51277 (Human)

Gene Symbol

DNAJC27

UniProt

Additional RBJ Products

Product Documents for RBJ Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RBJ Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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