RBM15 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82465

Novus Biologicals, part of Bio-Techne
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NBP1-82465
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RBM15 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DTYPPSASVVGASVGGHRHPPGGGGGQRSLSPGGAALGYRDYRLQQLALGRLPPPPPPPLPRDLERERDYPFYERVRPAYSLEPRVGAGAGAAPFREVDEISPEDDQRANRTLFL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RBM15 Antibody

Western Blot: RBM15 Antibody [NBP1-82465]

Western Blot: RBM15 Antibody [NBP1-82465]

Western Blot: RBM15 Antibody [NBP1-82465] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: RBM15 Antibody [NBP1-82465]

Immunocytochemistry/ Immunofluorescence: RBM15 Antibody [NBP1-82465]

Immunocytochemistry/Immunofluorescence: RBM15 Antibody [NBP1-82465] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RBM15 Antibody [NBP1-82465]

Immunohistochemistry-Paraffin: RBM15 Antibody [NBP1-82465]

Immunohistochemistry-Paraffin: RBM15 Antibody [NBP1-82465] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
View 6 more images

Applications for RBM15 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBM15

Members of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signalingpathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 (PubMed16129689)).(supplied by OMIM)

Alternate Names

FLJ12479, one twenty two protein, one twenty-two, One-twenty two protein 1, putative RNA-binding protein 15, RNA binding motif protein 15, RNA-binding motif protein 15, SPEN

Gene Symbol

RBM15

Additional RBM15 Products

Product Documents for RBM15 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RBM15 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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