Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RBM33 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 and 50 of human RNA Binding Motif Protein 33 using the numbering given in entry NP_444271.2 (GeneID 155435).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RBM33 Antibody

Western Blot: RBM33 Antibody [NBP2-22278]

Western Blot: RBM33 Antibody [NBP2-22278]

Western Blot: RBM33 Antibody [NBP2-22278] - Whole cell lysate from mouse NIH3T3 (50 ug), TCMK-1 (50 ug), and 4T1 (50 ug) cells. NBP2-22278 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: RBM33 Antibody [NBP2-22278]

Western Blot: RBM33 Antibody [NBP2-22278]

Western Blot: RBM33 Antibody [NBP2-22278] - Whole cell lysate from Jurkat (15 and 50 ug), HeLa (50 ug), and 293T (50 ug) cells. NBP2-22278 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: RBM33 Antibody [NBP2-22278]

Immunoprecipitation: RBM33 Antibody [NBP2-22278]

Immunoprecipitation: RBM33 Antibody [NBP2-22278] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-RBM33 antibody NBP2-22278 used for IP at 6 ug/mg lysate. RBM33 was inefficiently immunoprecipitated by rabbit anti-RBM33 antibodies NBP2-22279 and NBP2-22280. For blotting immunoprecipitated RBM33, NBP2-22280 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for RBM33 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RBM33

The RBM33 gene encodes a RNA-binding protein 33 that in isoform 1 is 1,170 amino acids long and nearly 130 kDA and in isoform 2 is 268 amino acids long at 30 kDA. RBM33 interacts with genes: TERF1, TERF2, GRB2, SUMO1, and SUMO3. It has been researched regarding its role in filariasis.

Alternate Names

DKFZp434D1319, MGC20460, proline-rich protein 8, RNA binding motif protein 33, RNA-binding motif protein 33, RNA-binding protein 33

Gene Symbol

RBM33

UniProt

NP_444271.2 (Human)

Additional RBM33 Products

Product Documents for RBM33 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RBM33 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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