RBM47 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83664

Novus Biologicals, part of Bio-Techne
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NBP1-83664
NBP1-83664-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Predicted:

Mouse (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RBM47 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HAMNNLNGTELEGSCLEVTLAKPVDKEQYSRYQKAARGGGAAEAAQQPSYVYSCDPYTLAYYGYPYNALIGPNRDYFVKAGSIRGRGRGAAGNRAPGPRGSYLGGYSAGRGIYSRYHEGKGKQQEKGYELVPNLEIPTVN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RBM47 Antibody

Immunohistochemistry-Paraffin: RBM47 Antibody [NBP1-83664]

Immunohistochemistry-Paraffin: RBM47 Antibody [NBP1-83664]

Immunohistochemistry-Paraffin: RBM47 Antibody [NBP1-83664] - Staining in human colon and skeletal muscle tissues using NBP1-83664 antibody. Corresponding RBM47 RNA-seq data are presented for the same tissues.
Western Blot: RBM47 Antibody [NBP1-83664]

Western Blot: RBM47 Antibody [NBP1-83664]

Western Blot: RBM47 Antibody [NBP1-83664] - Analysis in human cell lines A-549 and SK-MEL-30 using Anti-RBM47 antibody. Corresponding RBM47 RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.
Immunocytochemistry/ Immunofluorescence: RBM47 Antibody [NBP1-83664]

Immunocytochemistry/ Immunofluorescence: RBM47 Antibody [NBP1-83664]

Immunocytochemistry/Immunofluorescence: RBM47 Antibody [NBP1-83664] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
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Applications for RBM47 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBM47

The RBM47 gene encodes a RNA-binding protein 47 that in isoform 1 is 593 amino acid long, 64 kDA and in isoform 2 is 524 amino acid at 56 kDA. RBM47 interacts with genes ELAVL1 and ESR2. RBM47 is associated with intrahepatic cholangiocarcinoma.

Alternate Names

DKFZp686F02235, FLJ20273, FLJ21344, FLJ21643, NET18, RNA binding motif protein 47, RNA-binding motif protein 47, RNA-binding protein 47

Gene Symbol

RBM47

Additional RBM47 Products

Product Documents for RBM47 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RBM47 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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