RBM5 Antibody (2B6)

Novus Biologicals, part of Bio-Techne | Catalog # H00010181-M01

Novus Biologicals, part of Bio-Techne
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H00010181-M01
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2B6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RBM5 Antibodies »

Product Specifications

Immunogen

RBM5 (AAH02957, 75 a.a. ~ 184 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GYHSDGDYGEHDYRHDISDERESKTIMLRGLPITITESDIREMMESFEGPQPADVRLMKRKTGVSRGFAFVEFYHLQDATSWMEANQKKLVIQGKHIAMHYSNPRPKFED

Reactivity Notes

Human. Other species not tested.

Specificity

RBM5 - RNA binding motif protein 5

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for RBM5 Antibody (2B6)

Western Blot: RBM5 Antibody (2B6) [H00010181-M01]

Western Blot: RBM5 Antibody (2B6) [H00010181-M01]

Western Blot: RBM5 Antibody (2B6) [H00010181-M01] - Analysis of RBM5 over-expressed 293 cell line, cotransfected with RBM5 Validated Chimera RNAi ( Cat # H00010181-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RBM5 monoclonal antibody (M01), clone 2B6 (Cat # H00010181-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunocytochemistry/ Immunofluorescence: RBM5 Antibody (2B6) [H00010181-M01]

Immunocytochemistry/ Immunofluorescence: RBM5 Antibody (2B6) [H00010181-M01]

Immunocytochemistry/Immunofluorescence: RBM5 Antibody (2B6) [H00010181-M01] - Analysis of monoclonal antibody to RBM5 on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: RBM5 Antibody (2B6) [H00010181-M01]

Immunohistochemistry-Paraffin: RBM5 Antibody (2B6) [H00010181-M01]

Immunohistochemistry-Paraffin: RBM5 Antibody (2B6) [H00010181-M01] - Analysis of monoclonal antibody to RBM5 on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.
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Applications for RBM5 Antibody (2B6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RBM5

RNA-binding motif protein 5 (RBM5) is also known as LUCA-15. The RBM5 gene is located within a region highly susceptible to deletions in lung cancer and is implicated to be a tumor suppressor. RBM5 bears two RRM domains, one RanBP2-type zinc finger, one C2H2-type zinc finger, and a G-patch domain. It has been found to be a component of the spliceosome, a complex that regulates alternative splicing and is involved in the regulation of proliferation and apoptosis.

Alternate Names

G15, H37, LUCA15FLJ39876, Protein G15, Putative tumor suppressor LUCA15, Renal carcinoma antigen NY-REN-9, RMB5, RNA binding motif protein 5, RNA-binding motif protein 5, RNA-binding protein 5

Entrez Gene IDs

10181 (Human)

Gene Symbol

RBM5

OMIM

606884 (Human)

Additional RBM5 Products

Product Documents for RBM5 Antibody (2B6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RBM5 Antibody (2B6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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