RBX1 Antibody (SR1116)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21835

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21835-100ul
NBP3-21835-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1116

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RBX1 Antibodies »

Product Summary for RBX1 Antibody (SR1116)

Immunogen

A synthesized peptide derived from human RBX1 (Uniprot #: P62877)

Specificity

Detects endogenous levels of total RBX1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RBX1 Antibody (SR1116)

Western Blot: RBX1 Antibody (SR1116) [NBP3-21835] -

Western Blot: RBX1 Antibody (SR1116) [NBP3-21835] - Western blot analysis using NBP3-21835 in MCF7 cell lysate.

Applications for RBX1 Antibody (SR1116)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RBX1

ROC1 is a component of the SCF (SKP1-CUL1-F-box protein) and the CBC(VHL) (CUL2-elonging BC-VHL) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. ROC1 appears to recruit the E2 ubiquitination enzyme through the RING-type zinc finger in a manner similar to CDC34, and brings it into close proximity to the substrate. The RING-type zinc finger domain is essential for ubiquitin ligase activity. ROC1 probably also stimulates CDC34 autoubiquitination and promotes the neddylation of CUL1 and probably CUL2. ROC1 has a cytoplasmic and nuclear localization and is widely expressed in most tissues.

Long Name

Ring-Box 1

Alternate Names

Ring Box 1, RNF75, ZYP Protein

Gene Symbol

RBX1

Additional RBX1 Products

Product Documents for RBX1 Antibody (SR1116)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RBX1 Antibody (SR1116)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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