RCC1 Antibody (1F0C5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15295
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1F0C5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for RCC1 Antibody (1F0C5)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RCC1 (P18754). MSPKRIAKRRSPPADAIPKSKKVKVSHRSHSTEPGLVLTLGQGDVGQLGLGENVMERKKPALVSIPEDVVQAEAGGMHTVCLSKSGQVYSFGCNDEGALG
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RCC1 Antibody (1F0C5)
![Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295]](https://resources.bio-techne.com/images/products/RCC1-Antibody-1F0C5-Western-Blot-NBP3-15295-img0002.jpg "Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295]")
Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295]
Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295] - Analysis of extracts of various cell lines, using RCC1 Rabbit mAb (NBP3-15295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.![Immunohistochemistry-Paraffin: RCC1 Antibody (1F0C5) [NBP3-15295]](https://resources.bio-techne.com/images/products/RCC1-Antibody-1F0C5-Immunohistochemistry-Paraffin-NBP3-15295-img0001.jpg "Immunohistochemistry-Paraffin: RCC1 Antibody (1F0C5) [NBP3-15295]")
Immunohistochemistry-Paraffin: RCC1 Antibody (1F0C5) [NBP3-15295]
Immunohistochemistry-Paraffin: RCC1 Antibody (1F0C5) [NBP3-15295] - Human placenta using RCC1 Rabbit mAb (NBP3-15295) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.![Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295]](https://resources.bio-techne.com/images/products/RCC1-Antibody-1F0C5-Western-Blot-NBP3-15295-img0003.jpg "Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295]")
Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295]
Western Blot: RCC1 Antibody (1F0C5) [NBP3-15295] - Analysis of extracts of various cell lines, using RCC1 Rabbit mAb (NBP3-15295) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.Applications for RCC1 Antibody (1F0C5)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RCC1
Alternate Names
Cell cycle regulatory protein, CHC1RCC1-I, chromosome condensation 1, Chromosome condensation protein 1, guanine nucleotide-releasing protein, regulator of chromosome condensation, regulator of chromosome condensation 1, SNHG3-RCC1, SNHG3-RCC1 readthrough transcript
Gene Symbol
RCC1
Additional RCC1 Products
Product Documents for RCC1 Antibody (1F0C5)
Product Specific Notices for RCC1 Antibody (1F0C5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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