Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3D11

Concentration

LYOPH mg/ml

View all RCC1 Antibodies »

Product Summary for RCC1 Antibody (3D11)

Immunogen

Recombinant human RanGEF.

Reactivity Notes

Recognizes Human, Mouse and Rat RanGEF. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for RCC1 Antibody (3D11)

Western Blot: RCC1 Antibody (3D11) [NBP1-97543]

Western Blot: RCC1 Antibody (3D11) [NBP1-97543]

Western Blot: RCC1 Antibody (3D11) [NBP1-97543] - Analysis of RanGEF probed with RanGEF (RCC1), mAb (3D11).

Applications for RCC1 Antibody (3D11)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunoprecipitation

2 ug/ml

Western Blot

1 ug/ml
Application Notes
This antibody is useful in Immunocytochemistry, Immunoprecipitation and Western Blot (Detects a band of ~45kDa).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Reconstitute with 0.1 ml sterilized distilled water to a final concentration of 1.0 mg/ml.

Formulation

PBS (pH 7.2) and 1.0% Sucrose

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RCC1

RanGEF, also known as regulator of chromosome condensation (RCC1), is an abundant, highly conserved chromatin-associated 45 kDa protein. RanGEF is involved in the regulation of the onset of chromosome condensation in the S phase, and is the principle mammalian guanine nucleotide exchange factor (GEF) for the nuclear G protein Ran. RanGEF plays important roles in RNA transcription and processing, DNA replication, nuclear pore transport function, and cell cycle regulation.

Alternate Names

Cell cycle regulatory protein, CHC1RCC1-I, chromosome condensation 1, Chromosome condensation protein 1, guanine nucleotide-releasing protein, regulator of chromosome condensation, regulator of chromosome condensation 1, SNHG3-RCC1, SNHG3-RCC1 readthrough transcript

Gene Symbol

RCC1

Additional RCC1 Products

Product Documents for RCC1 Antibody (3D11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RCC1 Antibody (3D11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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