RCC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85637

Novus Biologicals, part of Bio-Techne
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NBP1-85637
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RCC1 Antibodies »

Product Summary for RCC1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MVPGKVELQEKVVQVSAGDSHTAALTDDGRVFLWGSFRDNNGVIGLLEPMKKSMVPVQVQLDVPVVKVASGNDHLVM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RCC1 Antibody

Western Blot: RCC1 Antibody [NBP1-85637]

Western Blot: RCC1 Antibody [NBP1-85637]

Western Blot: RCC1 Antibody [NBP1-85637] - Analysis using Anti-RCC1 antibody NBP1-85637 (A) shows similar pattern to independent antibody NBP1-85638 (B).
Immunocytochemistry/ Immunofluorescence: RCC1 Antibody [NBP1-85637]

Immunocytochemistry/ Immunofluorescence: RCC1 Antibody [NBP1-85637]

Immunocytochemistry/Immunofluorescence: RCC1 Antibody [NBP1-85637] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RCC1 Antibody [NBP1-85637]

Immunohistochemistry-Paraffin: RCC1 Antibody [NBP1-85637]

Immunohistochemistry-Paraffin: RCC1 Antibody [NBP1-85637] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.
View 3 more images

Applications for RCC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RCC1

Promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensationin the S phase. Binds to the chromatin. RCC1/Ran complex (together with other proteins) acts as a component of asignal transmission pathway that detects unreplicated DNA

Alternate Names

Cell cycle regulatory protein, CHC1RCC1-I, chromosome condensation 1, Chromosome condensation protein 1, guanine nucleotide-releasing protein, regulator of chromosome condensation, regulator of chromosome condensation 1, SNHG3-RCC1, SNHG3-RCC1 readthrough transcript

Gene Symbol

RCC1

Additional RCC1 Products

Product Documents for RCC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RCC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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