RCN1 Antibody (1F8-E4)

Novus Biologicals, part of Bio-Techne | Catalog # H00005954-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F8-E4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RCN1 Antibodies »

Product Specifications

Immunogen

RCN1 (AAH10120, 31 a.a. ~ 331 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PTVRKERVVRPDSELGERPPEDNQSFQYDHEAFLGKEDSKTFDQLTPDESKERLGKIVDRIDNDGDGFVTTEELKTWIKRVQKRYIFDNVAKVWKDYDRDKDDKISWEEYKQATYGYYLGNPAEFHDSSDHHTFKKMLPRDERRFKAADLNGDLTATREEFTAFLHPEEFEHMKEIVVLETLEDIDKNGDGFVDQDEYIADMFSHEENGPEPDWVLSEREQFNEFRDLNKDGKLDKDEIRHWILPQDYDHAQAEARHLVYESDKNKDEKLTKEEILENWNMFVGSQATNYGEDLTKNHDEL

Localization

Endoplasmic reticulum lumen.

Specificity

RCN1 - reticulocalbin 1, EF-hand calcium binding domain

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RCN1 Antibody (1F8-E4)

Western Blot: RCN1 Antibody (1F8-E4) [H00005954-M01]

Western Blot: RCN1 Antibody (1F8-E4) [H00005954-M01]

Western Blot: RCN1 Antibody (1F8-E4) [H00005954-M01] - RCN1 monoclonal antibody (M01), clone 1F8-E4. Analysis of RCN1 expression in HL-60.
Sandwich ELISA: RCN1 Antibody (1F8-E4) [H00005954-M01] - Detection limit for recombinant GST tagged RCN1 is approximately 1ng/ml as a capture antibody.

Applications for RCN1 Antibody (1F8-E4)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RCN1

Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding.

Alternate Names

FLJ37041, FLJ55835, PIG20, proliferation-inducing gene 20, RCAL, RCN, reticulocalbin 1, EF-hand calcium binding domain, reticulocalbin-1

Entrez Gene IDs

5954 (Human)

Gene Symbol

RCN1

OMIM

602735 (Human)

UniProt

AAH10120 (Human)

Additional RCN1 Products

Product Documents for RCN1 Antibody (1F8-E4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RCN1 Antibody (1F8-E4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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