Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RCN1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide that maps to a region between residues 1 and 75 of human Reticulocalbin-1, EF-hand calcium binding domain using the numbering given in Swiss-Prot entry Q15293 (GeneID 5954).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RCN1 Antibody

Western Blot: RCN1 Antibody [NB500-256]

Western Blot: RCN1 Antibody [NB500-256]

Western Blot: RCN1 Antibody [NB500-256] - Detection of human and mouse Reticulocalbin-1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Reticulocalbin-1 antibody NB500-256 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: RCN1 Antibody [NB500-256]

Immunohistochemistry-Paraffin: RCN1 Antibody [NB500-256]

Immunohistochemistry-Paraffin: RCN1 Antibody [NB500-256] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RCN1 (NB500-256). Detection: DAB
Immunoprecipitation: RCN1 Antibody [NB500-256]

Immunoprecipitation: RCN1 Antibody [NB500-256]

Immunoprecipitation: RCN1 Antibody [NB500-256] - Detection of human Reticulocalbin-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Reticulocalbin-1 antibody NB500-256 (lot NB500-256-3) used for IP at 6 ug per reaction. Reticulocalbin-1 was also immunoprecipitated by a previous lot of this antibody (lot NB500-256-2). For blotting immunoprecipitated Reticulocalbin-1, NB500-256 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for RCN1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:0000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RCN1

Reticulocalbin 1 is a calcium-binding protein located in the lumen of the ER. The protein contains six conserved regions with similarity to a high affinity Ca(+2)-binding motif, the EF-hand. High conservation of amino acid residues outside of these motifs, in comparison to mouse reticulocalbin, is consistent with a possible biochemical function besides that of calcium binding.

Alternate Names

FLJ37041, FLJ55835, PIG20, proliferation-inducing gene 20, RCAL, RCN, reticulocalbin 1, EF-hand calcium binding domain, reticulocalbin-1

Entrez Gene IDs

5954 (Human)

Gene Symbol

RCN1

UniProt

Q15293 (Human)

Additional RCN1 Products

Product Documents for RCN1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RCN1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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