RCOR1/CoREST Antibody (9B3R7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16225

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16225-100ul
NBP3-16225-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9B3R7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RCOR1/CoREST Antibodies »

Product Summary for RCOR1/CoREST Antibody (9B3R7)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RCOR1/CoREST (Q9UKL0). MPAMVEKGPEVSGKRRGRNNAAASASAAAASAAASAACASPAATAASGAAASSASAAAASAAAAPNNGQNKSLAAAAPNGNSSSNSWEEGSSGSSSDEEH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RCOR1/CoREST Antibody (9B3R7)

Western Blot: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225]

Western Blot: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225]

Western Blot: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Analysis of extracts of C2C12 cells, using RCOR1/CoREST Rabbit mAb (NBP3-16225) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225]

Immunohistochemistry-Paraffin: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225]

Immunohistochemistry-Paraffin: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Mouse brain using RCOR1/CoREST Rabbit mAb (NBP3-16225) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Chromatin Immunoprecipitation: RCOR1/CoREST Antibody (9B3R7) [NBP3-16225] - Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RCOR1/CoREST antibody (NBP3-16225) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
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Applications for RCOR1/CoREST Antibody (9B3R7)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

5ug antibody for 10ug-15ug of Chromatin

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RCOR1/CoREST

Brain type II sodium channels are important in neuronal phenotyping and are only seen at high levels in neurons. Studies have shown that CoREST functions as a corepressor for REST. This interaction between REST and CoREST is accomplished by means of a single zinc finger binding motif originating from REST. Together, the REST-CoREST complex mediates long term repression of the type II sodium channel gene (1).

Long Name

REST Corepressor 1

Alternate Names

CoREST, RCOR1

Gene Symbol

RCOR1

Additional RCOR1/CoREST Products

Product Documents for RCOR1/CoREST Antibody (9B3R7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RCOR1/CoREST Antibody (9B3R7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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