Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # HIS52

Concentration

0.5 mg/ml

View all RecA-1 Antibodies »

Product Specifications

Immunogen

Stromal cells from rat lymph node

Reactivity Notes

Does not react with:Goat, Chicken, Guinea Pig, Sheep, Mouse, Rabbit, Pig

Specificity

Recognizes RECA-1, a cell surface antigen which is expressed by all rat endothelial cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for RecA-1 Antibody (HIS52)

Immunohistochemistry-Frozen: RecA-1 Antibody (HIS52) - BSA Free [NB100-64647]

Immunohistochemistry-Frozen: RecA-1 Antibody (HIS52) - BSA Free [NB100-64647]

Immunohistochemistry-Frozen: RecA-1 Antibody (HIS52) [NB100-64647] - Used as a pan-endothelial marker to monitor the effect of CT treatment on the density of blood vessels in striatal explants.

Applications for RecA-1 Antibody (HIS52)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

<0.1% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RecA-1

RECA-1 reacts with rat endothelial cell antigen and showed to be reactive with all vascular endothelium in three tested rat strains of different MHC-haplotype; Lewis (TR-11), BN (RT-1n) and OA (RT-1u). RECA-1 recognizes a cell surface antigen. The antibody has been successfully applied in staining of viable endothelial cells in vitro, and of vascular endothelium in vivo. No reactivity of RECA-1 was seen with other cells e.g. fibroblasts, leukocytes and non endothelial stromal cells nor with other various tested species other than rat e.g. mouse, rabbit, sheep, goat and human. RECA-1 is a promising antibody for rat endothelial cell studies, and in particular for further defining nature and function of endothelial cell specific antigens.

Alternate Names

RecA protein, RecA1, RecA2, recaLP, Recombinase A

Additional RecA-1 Products

Product Documents for RecA-1 Antibody (HIS52)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for RecA-1 Antibody (HIS52)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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