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RECQL5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83438

Novus Biologicals, part of Bio-Techne
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NBP1-83438
NBP1-83438-25ul

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ELEHETFRNAKVANLYKASVLKKVADIHRASKDGQPYDMGGSAKSCSAQAEPPEPNEYDIPPASHVYSLKPKRVGAGFPKGSCPFQTATELMETTRIREQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RECQL5 Antibody

Western Blot: RECQL5 Antibody [NBP1-83438]

Western Blot: RECQL5 Antibody [NBP1-83438]

Western Blot: RECQL5 Antibody [NBP1-83438] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-RECQL5 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: RECQL5 Antibody [NBP1-83438]

Immunocytochemistry/ Immunofluorescence: RECQL5 Antibody [NBP1-83438]

Immunocytochemistry/Immunofluorescence: RECQL5 Antibody [NBP1-83438] - Staining of human cell line A-431 shows localization to nucleus & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RECQL5 Antibody [NBP1-83438]

Immunohistochemistry-Paraffin: RECQL5 Antibody [NBP1-83438]

Immunohistochemistry-Paraffin: RECQL5 Antibody [NBP1-83438] - Staining of human placenta shows no positivity in stromal cells as expected.

Applications for RECQL5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RECQL5

RECQL5 may have an important role in DNA metabolism

Alternate Names

DNA helicase, RecQ-like type 5, EC 3.6.1, EC 3.6.4.12, FLJ90603, RecQ protein-like 5ATP-dependent DNA helicase Q5, RecQ5, RECQ5RecQ protein 5

Gene Symbol

RECQL5

Additional RECQL5 Products

Product Documents for RECQL5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RECQL5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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